Viral Hemorrhagic Fever Panel Print

WSLH Department: CDD - Communicable Disease Division
WSLH Test Code: SA00100
Day(s) Performed:

Daily, STAT
Turn-around Time:

1 - 3 days
Recommended Uses:

Testing for patients with signs and/or symptoms of acute febrile illness and known or suspected exposure to viral hemorrhagic fever causing viruses. 

Evaluation for more common acute febrile illnesses should be considered and should include travel history, exposure risk, and consultation of the CDC yellow book prior to use of this panel.
Pre-approval:

Required prior to submission.

During business hours please contact CDD Customer Service at (800) 862-1013. 

After hours, please contact the 24/7 Emergency pager at (608) 263-3280

Additional pre-approval will also be required by the Wisconsin Division of Public Health (608) 258-0099 and CDC Viral Special Pathogens Branch.
Contraindications:

Specimens other than whole blood will be rejected.

Some pathogens in this assay have other preferred diagnostic testing methods and may be rejected for this test based on evaluation.
Additional Tests Performed:

Additional confirmatory testing may be automatically performed based on the testing algorithm.
Preparation for Collection:

Consultation on submission for this test will provide best collection practices.
Specimen Requirements:

Human whole blood collected in plastic EDTA tubes.

Two EDTA tubes requested. Minimum of 4 mL total.

Send immediately. Stable refrigerated (2-8°C) for up to 7 days.
Collection Instructions:

Specimen should be collected in plastic EDTA tubes. Do NOT separate plasma.
Collection Kit/Container:
Requisition Form:
Required Information:

Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers.
Rejection Criteria:

Improper sample type 

Samples collected > 7 days from receipt

Specimens received without pre-approval
Specimen Handling:
Transport specimens with frozen cold packs (2-8°C). Packaging instructions  
Packaging Requirements:
Courier Information:

Testing is eligible for STAT courier service upon testing approval.
Specimen Receiving Hours:

Monday-Friday 6:00 AM - 4:30 PM, Saturday 6:30 AM - 12:30 PM

Eligible for off-hours receiving
Results Include:

Detected, Not Detected or Invalid for each target
Limitations:

Positive results do not rule out co-infections with pathogens not included in the panel. 

Not all pathogens that cause acute febrile illness are detected by this test. 

Negative test results do not preclude infection with the pathogens targeted by this panel. Retesting 3 days after symptom onset may be necessary.
Additional Tests Recommended:
Additional Comments:

Results are intended to be used in conjunction with other clinical, epidemiologic and laboratory findings.
Methodology:

Multiplexed Real-time PCR
Includes:

Real-time PCR for the detection of Bacillus anthracis, Leptospira spp., Yersinia pestis, Francisella tularensis, Crimean-Congo hemorrhagic fever virus, Chikungunya virus, Dengue virus (serotypes 1, 2, 3 and 4), Lassa virus, Ebolavirus spp. (Bundibugyo, Reston, Sudan, Tai Forest, Zaire), Marburgvirus, West Nile Virus, Yellow fever virus, Plasmodium spp., Plasmodium falciparum, Plasmodium vivax/ovale, Leishmania spp. (including L. donovani and L. infantum).

Additional testing may be performed by the Wisconsin State Laboratory of Hygiene or CDC based on the results.
Regulatory Compliance:
CPT Code: 87797
Price: Fee exempt
Fee Exempt Eligible:

Yes, pre-approved testing is fee exempt
Billing and Revenue Office:

1-800-862-1065 arbill@slh.wisc.edu
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