Mpox (non-variola Orthopoxvirus) PCR
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | VR01720 |
| Day(s) Performed: | Monday-Friday |
| Turn-around Time: | 1-3 Days |
| Recommended Uses: | Detection of Mpox virus DNA in dermal lesion specimens from symptomatic individuals |
| Pre-approval: | No |
| Contraindications: | Dermal swab specimens MUST be collected from a dermal lesion. |
| Additional Tests Performed: |
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| Preparation for Collection: | |
| Specimen Requirements: | A dermal lesion material, including swabs of lesion surface, exudate or lesion crusts. Store specimens frozen (-20°C or lower) or refrigerated (2-8°C) promptly after collection. Send specimens immediately and within 7 days of collection. Minimum volume for testing is 1 mL |
| Collection Instructions: | Collect two swabs from each lesion, preferably from different locations on the body or from lesions that differ in appearance. Vigorously swab each lesion. Unroofing or aspiration of lesions is NOT necessary, nor recommended. Place swabs from lesions, crusts, exudate in separate tubes. Flocked swabs are preferred, but sterile Dacron, nylon, polyester or rayon swabs with plastic handles can also be used. Swabs may be placed in Viral Transport Medium (VTM). Dry swabs are also acceptable. Do not use cotton swabs. |
| Collection Kit/Container: |
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| Requisition Form: |
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| Required Information: | Include patient Mpox vaccination status and dates of vaccination on the requisition form, if available. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers. |
| Rejection Criteria: | Improper specimen type (e.g. a non-lesion dermal swab, exudate or crust) Specimens collected with an unacceptable swab type (e.g. cotton or calcium alginate swabs, swabs with wooden handles). Swabs placed in unacceptable transport media (e.g. Amies media, UTM). Specimens received >7 days after collection. Specimens transported without proper coolant. |
| Specimen Handling: | Transport specimens at 2-8°C with a frozen cold pack or on dry ice if specimen is frozen. |
| Packaging Requirements: | |
| Courier Information: | This test is eligible for fee-exempt courier service upon pre-approval only. Contact Purple Mountain Dispatch at 800-990-9668 for specimen pickup. |
| Specimen Receiving Hours: | Monday-Friday 6:00 AM - 4:30 PM, Saturday 6:30 AM - 12:30 PM |
| Results Include: | No Non-variola orthopox virus DNA detected Non-variola orthopox virus DNA detected Indeterminate |
| Limitations: | A negative test result does not rule out infection with Mpox. |
| Additional Tests Recommended: | |
| Additional Comments: |
| Methodology: | Real-time PCR |
| Includes: | Real-time PCR for the detection and strain typing of Mpox (non-variola Orthopoxvirus) DNA |
| Regulatory Compliance: |
| CPT Code: | 87798 |
| Price: | $215.00 |
| Fee Exempt Eligible: | Yes. If fee-exempt testing at the WSLH is needed, please contact DHS at 608-258-0099. |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |