Streptococcus pneumoniae serotyping
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP000689 |
| Day(s) Performed: | Monday-Friday |
| Turn-around Time: | 10 business days |
| Recommended Uses: | For epidemiologic purposes to support WDPH to identify strains associated with invasive disease. |
| Pre-approval: | N |
| Contraindications: | |
| Additional Tests Performed: |
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| Preparation for Collection: | |
| Specimen Requirements: | Streptococcus pneumonia isolates: ▪ From CSF ▪ Resistant to any clinically relevant antibiotic ▪ Suspect treatment failure ▪ Suspect vaccine failure |
| Collection Instructions: | |
| Collection Kit/Container: |
Isolate on chocolate agar or blood agar
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| Requisition Form: |
Streptococcus pneumonia requisition form
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Rejection Criteria: | Mixed cultures may significantly lengthen turnaround time. |
| Specimen Handling: | Isolates may be stored and transported at ambient temperatures |
| Packaging Requirements: | |
| Courier Information: | |
| Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Results Include: | Streptococcus pneumonia serotype information |
| Limitations: | Cultures must be viable for identification. It may not always be possible to discriminate between multiple serotypes within a serogroup due to a high degree of genetic similarities or due to the existence of DNA polymorphisms among single serotypes. Therefore, a serogroup will be assigned for isolates where a serotype cannot be identified. Isolates that are not able to be serotyped will be sent to CDC for further identification. |
| Additional Tests Recommended: | |
| Additional Comments: |
| Methodology: | Whole Genome Sequencing |
| Includes: | Confirmation of bacterial identification and serotyping |
| Regulatory Compliance: |
| CPT Code: | 87999 |
| Price: | $125.00 |
| Fee Exempt Eligible: | N |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |