Streptococcus pneumoniae Characterization Print

WSLH Department: CDD - Communicable Disease Division
WSLH Test Code: MP00689
Day(s) Performed:

Monday-Friday

Turn-around Time:

10-14 days

Recommended Uses:

For public health surveillance of strains associated with invasive disease

Pre-approval:

No

Contraindications:

Non-invasive isolates

Additional Tests Performed:
Preparation for Collection:
Specimen Requirements:

All CSF isolates

Streptococcus pneumoniae invasive isolates that are: 

Resistant to any clinically relevant antibiotic

Suspected vaccine failure

Suspected treatment failure

Suspected outbreak

Collection Instructions:
Collection Kit/Container:

Isolate on chocolate agar or blood agar

Requisition Form:
Required Information:

Vaccine history and susceptibility results

Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers.

Rejection Criteria:

Isolates that do not meet surveillance criteria

Specimen Handling:

Isolates may be stored and transported at ambient temperatures

Packaging Requirements:
Courier Information:

Specimens submitted for public health surveillance are eligible for fee-exempt courier service. Contact Purple Mountain Dispatch at 800-990-9668 for specimen pick up (in Wisconsin) or use the VPD FedEx number if sending from out of state.

Specimen Receiving Hours:

Monday-Friday 6:00 AM - 4:30 PM, Saturday 6:30 AM - 12:30 PM

Results Include:

Streptococcus pneumoniae serotype

Limitations:

Cultures must be viable for identification. Mixed cultures may significantly lengthen turnaround time.  It may not always be possible to discriminate between multiple serotypes within a serogroup due to a high degree of genetic similarities or due to the existence of DNA polymorphisms among single serotypes. Therefore, a serogroup will be assigned for isolates where a serotype cannot be identified. Isolates that are not able to be serotyped may be sent to a reference lab for further identification at no additional charge.

Additional Tests Recommended:
Additional Comments:
Methodology:

Whole Genome Sequencing

Includes:

Bacterial isolate identification confirmation, genotyping/serotyping of isolates

Regulatory Compliance:
CPT Code: 87999
Price: $133.00
Fee Exempt Eligible:

Yes, for isolates meeting the public health surveillance request

Billing and Revenue Office:

1-800-862-1065 arbill@slh.wisc.edu

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