Neisseria meningitidis PCR
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP00561 |
| Day(s) Performed: | Monday - Friday |
| Turn-around Time: | 1-3 days |
| Recommended Uses: | Detection of Neisseria meningitidis CNS infections from Cerebrospinal Fluid; to serogroup strains of Neisseria meningitidis associated with invasive disease for public health surveillance. |
| Pre-approval: | No |
| Contraindications: | Asymptomatic individuals |
| Additional Tests Performed: |
If positive for SodC gene marker, Neisseria meningitidis Serogrouping PCR (test code MP00563) will be auto-reflexed at no additional charge. Antimicrobial susceptibility testing. Please note antimicrobial susceptibility testing is performed for surveillance purposes only, no report will be provided. |
| Preparation for Collection: | |
| Specimen Requirements: | Cerebrospinal Fluid: 1.0 mL (200 µL minimum) Isolate: N. meningitidis isolate collected from sterile body site |
| Collection Instructions: | |
| Collection Kit/Container: |
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| Requisition Form: |
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers. |
| Rejection Criteria: | Isolates from non-invasive sources |
| Specimen Handling: | Store refrigerated (2-8°C) or frozen (-20°C). Ship with frozen cold packs or dry ice. |
| Packaging Requirements: | |
| Courier Information: | Specimens and isolates submitted for public health surveillance or outbreak investigations are eligible for fee-exempt courier service. Contact Purple Mountain Dispatch at 800-990-9668 for specimen pick up (in Wisconsin) or use the VPD FedEx number if sending from out of state. |
| Specimen Receiving Hours: | Monday-Friday 6:00 AM - 4:30 PM, Saturday 8:00 AM - 12:00 PM |
| Results Include: | Neisseria meningitidis DNA detected/not detected; Neisseria meningitidis serogroup/Unable to serogroup |
| Limitations: | A negative test result does not rule out infection with N. meningitidis. Neisseria meningitidis Serogrouping PCR is limited to Serogroups A, B, C, W135, X, and Y. |
| Additional Tests Recommended: | |
| Additional Comments: | One positive isolate or CSF per patient per year is requested for public heath surveillance |
| Methodology: | Real-Time PCR |
| Includes: | Detection/confirmation of the SodC gene for species specific detection of Neisseria meningitidis from CSF and bacterial isolates, respectively; serogrouping |
| Regulatory Compliance: |
| CPT Code: | 87798 |
| Price: | $225.00 |
| Fee Exempt Eligible: | Yes, if submitted for public health surveillance or outbreak investigations |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |