Streptococcus pneumoniae PCR
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP00461 |
| Day(s) Performed: | Monday-Friday |
| Turn-around Time: | 1-3 days |
| Recommended Uses: | Detection of Streptococcus pneumoniae in cerebrospinal fluid when culture is negative and/or patient has been treated with antibiotics prior to specimen collection (e.g., isolate recovery is unlikely). |
| Pre-approval: | No |
| Contraindications: | Asymptomatic individuals |
| Additional Tests Performed: |
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| Preparation for Collection: | |
| Specimen Requirements: | Cerebrospinal Fluid-1.0 mL (200 µL minimum) |
| Collection Instructions: | |
| Collection Kit/Container: |
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| Requisition Form: |
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers. |
| Rejection Criteria: | |
| Specimen Handling: | Refrigerated (2-8°C) or frozen (-20°C) prior to shipment. Ship with frozen cold packs or dry ice. |
| Packaging Requirements: | Refrigerated (2-8°C) or frozen (-20°C) prior to shipment. Ship with frozen cold packs or dry ice. |
| Courier Information: | Specimens submitted for public health surveillance are eligible for fee-exempt courier service. Use the Purple Mountain portal for specimen pick up (in Wisconsin) or use the VPD FedEx account if sending from out of state. |
| Specimen Receiving Hours: | Monday-Friday 6:00 AM - 4:30 PM, Saturday 8:00 AM - 12:00 PM |
| Results Include: | Streptococcus pneumoniae DNA detected/not detected |
| Limitations: | A negative test result does not rule out infection with S. pneumoniae. Serotyping cannot be performed from CSF. |
| Additional Tests Recommended: | |
| Additional Comments: |
| Methodology: | Real-Time PCR |
| Includes: | Detection of the lyta gene for species specific detection of Streptococcus pneumoniae from CSF. |
| Regulatory Compliance: |
| CPT Code: | 87798 |
| Price: | $300.00 |
| Fee Exempt Eligible: | Yes, for specimens meeting the public health surveillance request |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |