Shiga toxin PCR
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP00545 |
| Day(s) Performed: | Weekly |
| Turn-around Time: | 1-7 days |
| Recommended Uses: | 1. Detection of Shiga toxin-producing bacteria in stool specimens from cases of community-acquired diarrhea and cases with suspected or confirmed hemolytic uremic syndrome (HUS). 2. Confirmation of Shiga toxin EIAs or other other testing method (i.e. molecular syndromic panels). |
| Pre-approval: | N |
| Contraindications: | |
| Additional Tests Performed: |
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| Preparation for Collection: | |
| Specimen Requirements: | ▪ Stool specimens in Cary Blair transport media ▪ Bacterial isolates ▪ Enrichment broth (e.g. GN or MacConkey broth) |
| Collection Instructions: | Collect stool specimens according to instructions in Kit #10. Refer to instructions used at your facility for proper inoculation and handling of enrichment broths. |
| Collection Kit/Container: |
Stool Culture (Enteric Pathogen Culture)
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| Requisition Form: |
CDD Requisition Form A
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Rejection Criteria: | Unpreserved stool specimen |
| Specimen Handling: | ▪ Store stools at 2-8 degrees Celsius and transport with cool pack. ▪ After incubation, store enrichment broths at 2-8 degrees Celsius C and transport with cool pack. ▪ Isolates may be stored and transported at ambient temperatures |
| Packaging Requirements: | |
| Courier Information: | |
| Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Results Include: | Shiga toxin 1 DNA detected/ not detected; Shiga toxin 2 DNA detected/ not detected; eae DNA detected/not detected; ehxA DNA detected/not detected |
| Limitations: | |
| Additional Tests Recommended: | Routine stool culture for enteric bacterial pathogens, including E. coli O157 |
| Additional Comments: |
| Methodology: | Real-time PCR |
| Includes: | Real-time PCR for the detection of Shiga toxin 1 and Shiga toxin 2 genetic markers from cultures of stool specimens. |
| Regulatory Compliance: |
| CPT Code: | 87798 |
| Price: | $477.00 Price Effective 1/1/25 |
| Fee Exempt Eligible: | N |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |