Weekly

1-7 days

Detection of Shiga toxin-producing bacteria in stool specimens from cases of community-acquired diarrhea and cases with suspected or confirmed hemolytic uremic syndrome (HUS). 

Confirmation of Shiga toxin EIAs or other testing method (i.e. molecular syndromic panels).

No

Asymptomatic individuals

Whole genome sequencing is automatically performed on all recovered isolates for public health surveillance. There will be no additional charge and no report to the submitter for this testing.

Stool specimens in Cary Blair transport media

Bacterial isolates

Enrichment broth (e.g. GN or MacConkey broth)

Collect stool specimens according to instructions in Kit #10. Refer to instructions used at your facility for proper inoculation and handling of enrichment broths

10 Stool Culture (Enteric Pathogen Culture)

CDD Requisition Form A

Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers.

Unpreserved stool specimen

Stools or enrichment broths should be stored refrigerated (2-8°C) and transported with frozen cold packs. 

Isolates may be stored and transported at ambient temperatures.

Cultures and isolates that are known to contain Shiga-toxin producing E. coli are Category A infectious substances and must be packaged according to current regulations. 

Unknowns may be packaged as Category B depending on your lab’s procedures.

Specimens, cultures, and isolates submitted for public health surveillance or outbreak investigations are eligible for fee-exempt courier service. Contact Purple Mountain Dispatch at 800-990-9668 for specimen pick up.

Monday-Friday 6:00 AM - 4:30 PM, Saturday 6:30 AM - 12:30 PM

Shiga toxin 1 DNA detected/ not detected; Shiga toxin 2 DNA detected/ not detected; eae DNA detected/not detected; ehxA DNA detected/not detected

One positive isolate, stool, or enrichment broth per patient per year is requested for public heath surveillance.

Real-time PCR and Culture

Real-time PCR for the detection of Shiga toxin 1 and Shiga toxin 2 genetic markers from isolates or stool specimens. Culture is performed on all submitted material.

Laboratory Developed Test (LDT)

Yes, if submitted for public health surveillance or outbreak investigations

1-800-862-1065 arbill@slh.wisc.edu