Francisella tularensis Culture
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP00400 |
| Day(s) Performed: | Monday - Friday |
| Turn-around Time: | 3-7 days |
| Recommended Uses: | Identification of Francisella tularensis in individuals exhibiting symptoms consistent with tularemia. |
| Pre-approval: | Yes, please contact CDD Customer Service at (800)-862-1013 prior to submission |
| Contraindications: | |
| Additional Tests Performed: |
Isolates that are unable to be ruled out as a Select Agent will be automatically reflexed to PCR and DFA confirmation at no additional charge. |
| Preparation for Collection: | |
| Specimen Requirements: | Culture isolates or synthetic swab of lesion. For high suspect patient: positive blood culture bottles (blood and bone marrow); sterile vial with fluid specimens other than blood and bone marrow; sterile vial with non-bacteriostatic saline for tissues. |
| Collection Instructions: | Aseptic collection of specimens |
| Collection Kit/Container: |
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| Requisition Form: |
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| Required Information: | Please include all pertinent clinical information, lab rule-out testing results, and exposure history. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers. |
| Rejection Criteria: | Formalinized or fixed tissue is unacceptable for culture |
| Specimen Handling: | Store specimens (other than blood culture bottles) in a refrigerator (2-8°C) and ship with frozen cold packs. Ship blood culture bottles at ambient temperatures. It is recommended that specimens are received in the laboratory within 24-48 hours of collection. |
| Packaging Requirements: | |
| Courier Information: | Contact Purple Mountain Dispatch at 800-990-9668 for specimen pick up. |
| Specimen Receiving Hours: | Monday-Friday 6:00 AM - 4:30 PM, Saturday 6:30 AM - 12:30 PM |
| Results Include: | Negative for F. tularensis, Presumptive Francisella tularensis, or Francisella tularensis detected |
| Limitations: | |
| Additional Tests Recommended: | Francisella tularensis Serology |
| Additional Comments: | If Francisella is suspected, please call the WSLH prior to submission of clinical specimens. Blood cultures should be incubated at the clinical laboratory. Francisella tularensis is a Select Agent under Federal Regulations and must be reported to the CDC Select Agent Program. Enhanced precautions should be taken when handling suspect specimens or isolates. |
| Methodology: | Bacterial culture and biochemical characterization, PCR and DFA confirmation of positives |
| Includes: | Identification of Fransicella tularensis isolates |
| Regulatory Compliance: |
| CPT Code: | 87081 |
| Price: | $61.00 |
| Fee Exempt Eligible: | Yes |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |