Legionella RT-PCR Test Print
WSLH Department: | CDD - Communicable Disease Division |
WSLH Test Code: | MP00441 |
Availability: | Monday-Friday |
Turn-around Time: | 1-3 days |
Recommended Uses: | Detection of Legionella DNA for identification and/or confirmation; species identification and serogrouping of isolates for epidemiological purposes. |
Contraindications: | This test is for clinical use only, not for use with environmental samples. |
Additional Tests Performed: |
Patient Preparations: | |
Specimen Requirements: | Sputum or BAL of suspect patients collected in sterile container. Pure bacterial isolate of suspected Legionella sp. on BCYE agar. |
Specimen Handling & Transport: | • Primary specimens can be stored at 2-8C until transport and transported with a cool pack. Primary specimens must be tested within 3 days of collection per CDC guidance. If specimens will not be tested for 3 or more days, they should be stored at -20°C until ready for shipment and shipped on dry ice. • Isolates can be stored at ambient temperatures; avoid freezing. |
Collection Kit/Container: |
TB Sputum/Legionella
|
Collection Instructions: | |
Unacceptable Conditions: | |
Requisition Form: |
CDD Requisition Form A
|
Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
Results Include: | Legionella DNA detected/not detected Legionella pneumophila DNA detected/not detected Legionella pneumophila serogroup 1 DNA detected/not detected |
Limitations: | This test was developed and its performance characteristics determined by the Wisconsin State Laboratory of Hygiene. It has not been cleared by the U.S. Food and Drug Administration. A negative result does not rule out Legionella infection. |
Additional Tests Recommended: | Legionella Culture (primary specimens); Legionella Identification (isolates) |
Additional Comments: |
Methodology: | Real-time PCR |
Includes: | Real-time PCR for the detection of Legionella species and serogrouping of Legionella pneumophila from primary specimens and bacterial isolates. |
CPT Code: | 87541, 2x 87798 |
Price: | $165.00 |