Legionella RT-PCR Test
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP00441 |
| Day(s) Performed: | Monday-Friday |
| Turn-around Time: | 1-3 days |
| Recommended Uses: | Detection of Legionella DNA for identification and/or confirmation; species identification and serogrouping of isolates for epidemiological purposes. |
| Contraindications: | This test is for clinical use only, not for use with environmental samples. |
| Additional Tests Performed: |
| Patient Preparations: | |
| Specimen Requirements: | Sputum or BAL of suspect patients collected in sterile container. Pure bacterial isolate of suspected Legionella sp. on BCYE agar. |
| Specimen Handling & Transport: | � Primary specimens can be stored at 2-8C until transport and transported with a cool pack. Primary specimens must be tested within 3 days of collection per CDC guidance. If specimens will not be tested for 3 or more days, they should be stored at -20�C until ready for shipment and shipped on dry ice. � Isolates can be stored at ambient temperatures; avoid freezing. |
| Collection Kit/Container: |
TB Sputum/Legionella
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| Collection Instructions: | |
| Unacceptable Conditions: | |
| Requisition Form: |
CDD Requisition Form A
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Legionella DNA detected/not detected Legionella pneumophila DNA detected/not detected Legionella pneumophila serogroup 1 DNA detected/not detected |
| Limitations: | This test was developed and its performance characteristics determined by the Wisconsin State Laboratory of Hygiene. It has not been cleared by the U.S. Food and Drug Administration. A negative result does not rule out Legionella infection. |
| Additional Tests Recommended: | Legionella Culture (primary specimens); Legionella Identification (isolates) |
| Additional Comments: |
| Methodology: | Real-time PCR |
| Includes: | Real-time PCR for the detection of Legionella species and serogrouping of Legionella pneumophila from primary specimens and bacterial isolates. |
| CPT Code: | 87541, 2x 87798 |
| Price: | $175.00 Price Effective 1/1/25 |