BCL2 Rearrangement, FISH Print

WSLH Department: Cytogenetics
WSLH Test Code: 882F96
Availability: Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time: Approximately 4-6 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses: Detection of rearrangements of the BCL2 gene associated with B-cell lymphomas including follicular lymphoma and diffuse large B-cell lymphoma ("double-hit" or "triple-hit"). Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant. May be performed in conjunction with other FISH assays, chromosome analysis (for bone marrow, blood, and fresh tissue only) or as an independent test.
Additional Tests Performed:
Patient Preparations:
Specimen Requirements: Paraffin embedded tissue: Five 4-micron sections on immunohistochemistry slides, the middle section stained with H&E.
Specimen Handling & Transport: Store and transport specimens at room temperature.
Collection Kit/Container:
Collection Instructions: FFPE tissue: Tissue specimen should be fixed in 10% neutral buffered formalin for 6-72 hours.
Unacceptable Conditions: FFPE tissue specimen must not be de-calcified.
Requisition Form: Cytogenetics Lab Neoplasia Diagnosis Form #132
Required Information: Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results Include: Number of nuclei examined, percent positive for BCL2 rearrangement, and the reference range for false positives based on the number of cells with an abnormal signal pattern in control cells. Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results.
Limitations: Detects rearrangements involving this gene; the translocation partner chromosome cannot be determined by this test. Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen. This probe will not detect small deletions or duplications or base pair changes.
Additional Tests Recommended: IGH/BCL2 Fusion, t(14;18)(q32;q21), FISH BCL6 Rearrangement, FISH MYC Rearrangement, FISH Chromosome Analysis, Bone Marrow, for Hematologic Disorders
Additional Comments:
Methodology: Dual color FISH analysis was performed on interphase nuclei (nuc ish) using a break-apart DNA probe (LSI BCL2 dual color, rearrangement; Abbott Molecular) specific for the BCL2 gene in 18q21. This probe is designed to detect rearrangements of the BCL2 gene including translocations and deletions. Translocation partner chromosomes cannot be determined by this test.
Includes: FISH analysis of 200 interphase cells for rearrangement of the BCL2 gene
CPT Code: 88271x2, 88275
Price: For pricing information, please call 608-262-0402.
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