Rubella Virus PCR
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WSLH Department: | CDD - Communicable Disease Division |
WSLH Test Code: | VR01725 |
Day(s) Performed: | Daily, Monday - Friday |
Turn-around Time: | 1-3 Days |
Recommended Uses: | Detection of rubella infection in symptomatic individuals |
Pre-approval: | Yes - Please contact the Wisconsin Immunization Program at 608-267-9959 or after hours at 608-258-0099 for approval |
Contraindications: | Asymptomatic individuals |
Additional Tests Performed: |
Preparation for Collection: | Detection is most successful when samples are collected the first day of rash through 3 days following rash onset. Detection may be successful as late as 7 days post rash onset. For samples collected from suspected congenital rubella syndrome (CRS) cases, the collection window is from birth to 3 months of age. |
Specimen Requirements: | A nasopharyngeal swab (preferred), oropharyngeal (throat) swab, or combined nasopharyngeal/oropharyngeal swab, placed in viral transport media is acceptable for testing. Neat urine is also acceptable. Store specimens refrigerated (2-8 °C). Send specimens immediately and within 10 days of collection. Minimum volume for testing is 1mL (15mL is the minimum for urine specimens). |
Collection Instructions: | Specimens should be collected with a Dacron or polyester swab and placed in Viral Transport Medium (VTM) or Universal Transport Medium (UTM). Urine should be collected in a sterile, leak proof container. |
Collection Kit/Container: |
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Requisition Form: |
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Required Information: | Include patient MMR vaccination status and dates of vaccination on the requisition form, if available. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
Rejection Criteria: | Improper specimen type (e.g. Nasal swab, buccal swab). Specimens collected with an unacceptable swab type (e.g. cotton or calcium alginate swabs or swabs with wooden sticks). Dry swabs, or swabs placed in unacceptable transport media (e.g. Amies media). Specimens collected >10 days from receipt. Specimens transported without proper coolant. |
Specimen Handling: | Transport specimens at 2-8 °C with a frozen cold pack |
Packaging Requirements: | |
Courier Information: | This test is eligible for fee-exempt courier service. Contact Purple Mountain Dispatch at 800-990-9668 for specimen pickup. |
Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
Results Include: | Rubella RNA detected/not detected, Equivocal, Indeterminate |
Limitations: | A negative test result does not rule out infection with rubella virus. |
Additional Tests Recommended: | |
Additional Comments: | Please contact the Wisconsin Immunization Program at 608-267-9959 or after hours at 608-258-0099 for approval when submitting specimens for rubella virus testing. |
Methodology: | Real-time RT-PCR |
Includes: | Real-time RT-PCR for detection of rubella virus RNA in symptomatic individuals. If positive for rubella virus RNA, rubella virus genotyping will be performed for epidemiologic surveillance purposes. |
Regulatory Compliance: |
CPT Code: | 87798 |
Price: | $225.00 |
Fee Exempt Eligible: | Yes; If fee-exempt testing at the WSLH is needed, please contact the Wisconsin Immunization Program at 608-267-9959 or after hours at 608-258-0099 |
Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |