Rickettsia IgG Antibody
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | SS02042 |
| Day(s) Performed: | Test is performed weekly |
| Turn-around Time: | 1-8 days |
| Recommended Uses: | Serodiagnosis of an infection with one of these agents. Paired acute and convalescent sera are preferred for diagnostic testing. |
| Pre-approval: | N |
| Contraindications: | |
| Additional Tests Performed: |
| Preparation for Collection: | Routine blood draw. |
| Specimen Requirements: | 1-3 ml serum |
| Collection Instructions: | Routine blood draw. |
| Collection Kit/Container: |
Serology Specimens
Sterile Transfer Tube |
| Requisition Form: |
CDD Requisition Form B
|
| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Rejection Criteria: | Obvious microbial contamination, hemolyzed, or lipemic sera |
| Specimen Handling: | Store serum specimens at 2-8 degrees C and transport with cool pack. |
| Packaging Requirements: | |
| Courier Information: | |
| Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Results Include: | RMSF Titer and Typhus Titer. Diagnostically Significant Titer: Four-fold titer change with convalescent titer greater than or equal to 1:128; single serum titer of greater than or equal to 1:256. |
| Limitations: | Limitations: Some cross reactivity between Rocky Mountain Spotted Fever and Murine Typhus occurs at lower dilutions. |
| Additional Tests Recommended: | Patients returning from rickettsial endemic areas with antibody reactivity to the Spotted Fever Group rickettsia and/or Typhus Group rickettsia should be tested for Orientia tsutsugamushi (scrub typhus) to rule out the possibility of serological cross-reactivity. |
| Additional Comments: |
| Methodology: | Indirect Fluorescent Antibody Test (IFA) |
| Includes: | Semi-quantitative total antibody IFA testing for Rocky Mountain Spotted Fever and Murine Typhus. |
| Regulatory Compliance: |
| CPT Code: | 86757x2 |
| Price: | $61.00 Price Effective 1/1/25 |
| Fee Exempt Eligible: | N |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |