Chromosome Analysis, Chorionic Villus Sample-Abridged, for Pre-natal Genetic Diagnosis
| WSLH Department: | Cytogenetics |
| WSLH Test Code: | 857 |
| Availability: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | Approximately 7-14 days, with an average of 13 days (longer for specimens with small volume or poor growth). (Reports are issued Monday-Friday 7:45 AM - 4:30 PM) |
| Recommended Uses: | Abridged chromosome analysis to be ordered in conjunction with Prenatal Microarray (Comprehensive or Targeted) for a fetus with one or more major structural abnormalities identified on ultrasonographic examination. |
| Contraindications: | |
| Additional Tests Performed: |
Illumina Microarray Analysis
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| Patient Preparations: | |
| Specimen Requirements: | 10-30mg chorionic villus sample (will be used for both the Abridged chromosome analysis and the Prenatal Microarray) |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. Specimens must be received by the laboratory within 24 hours of collection. |
| Collection Kit/Container: |
Cytogenetics and Molecular Genetics Collection Kit
|
| Collection Instructions: | Using aseptic technique, obtain at least 10mg of chorionic villi, taken between 11-38 weeks of gestation. Contact the laboratory (608-262-0402) to order CVS kits. [Kits include: 1 T-25 flask with 10ml F10 and 0.2ml sodium heparin, 1 test request form (#131), 1 Biohazard bag and absorbent pad. Store kits @4C for up to 1 year If requested a UPS return label can be supplied with the kit.] |
| Unacceptable Conditions: | A specimen with no fetal material identified and only maternal decidua present will be rejected. |
| Requisition Form: |
Cytogenetics Lab Genetic Diagnosis Form #131
|
| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, gestational age, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results. |
| Limitations: | The cytogenetic methods used in this analysis do not routinely detect small structural rearrangements, microdeletions, or low level (<38%) mosaicism. |
| Additional Tests Recommended: | |
| Additional Comments: |
| Methodology: | Microscopic analysis of G-banded chromosomes. |
| Includes: | G-banded chromosome analysis of cultured cells from chorionic villus sample (CVS). Includes in situ culture of cells from chorionic villus, examination of 5 metaphase cells from 5 independent colonies, and preparation of 1 karyogram. |
| CPT Code: | 88235, 88261 |
| Price: | For pricing information, please call 608-262-0402. |