Chromosome Analysis, Chorionic Villus Sample-Abridged, for Pre-natal Genetic Diagnosis

WSLH Department: Cytogenetics
WSLH Test Code: 857
Availability: Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time: Approximately 7-14 days, with an average of 13 days (longer for specimens with small volume or poor growth). (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses: Abridged chromosome analysis to be ordered in conjunction with Prenatal Microarray (Comprehensive or Targeted) for a fetus with one or more major structural abnormalities identified on ultrasonographic examination.
Additional Tests Performed: Illumina Microarray Analysis
Patient Preparations:
Specimen Requirements: 10-30mg chorionic villus sample (will be used for both the Abridged chromosome analysis and the Prenatal Microarray)
Specimen Handling & Transport: Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. Specimens must be received by the laboratory within 24 hours of collection.
Collection Kit/Container: Cytogenetics and Molecular Genetics Collection Kit
Collection Instructions: Using aseptic technique, obtain at least 10mg of chorionic villi, taken between 11-38 weeks of gestation.
Contact the laboratory (608-262-0402) to order CVS kits.
[Kits include: 1 T-25 flask with 10ml F10 and 0.2ml sodium heparin, 1 test request form (#131), 1 Biohazard bag and absorbent pad. Store kits @4C for up to 1 year If requested a UPS return label can be supplied with the kit.]
Unacceptable Conditions: A specimen with no fetal material identified and only maternal decidua present will be rejected.
Requisition Form: Cytogenetics Lab Genetic Diagnosis Form #131
Required Information: Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, gestational age, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results Include: Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results.
Limitations: The cytogenetic methods used in this analysis do not routinely detect small structural rearrangements, microdeletions, or low level (<38%) mosaicism.
Additional Tests Recommended:
Additional Comments:
Methodology: Microscopic analysis of G-banded chromosomes.
Includes: G-banded chromosome analysis of cultured cells from chorionic villus sample (CVS). Includes in situ culture of cells from chorionic villus, examination of 5 metaphase cells from 5 independent colonies, and preparation of 1 karyogram.
CPT Code: 88235, 88261
Price: For pricing information, please call 608-262-0402.
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