Chromosome Analysis, Amniotic Fluid-Abridged, for Pre-natal Genetic Diagnosis

WSLH Department: Cytogenetics
WSLH Test Code: 852
Availability: Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time: Approximately 7-14 days, with an average of 11 days (longer for specimens with small volume, containing blood or slow growth). (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses: Abridged chromosome analysis to be ordered in conjunction with Prenatal Microarray (Comprehensive or Targeted) for a fetus with one or more major structural abnormalities identified on ultrasonographic examination.
Additional Tests Performed:
Patient Preparations:
Specimen Requirements: 15-30 ml amniotic fluid (will be used for both the Abridged chromosome analysis and the Prenatal Microarray)
Specimen Handling & Transport: Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 hours of collection.
Collection Kit/Container:
Collection Instructions: Collect the fluid under sterile, ultrasound guided conditions using a 22-gauge needle inserted through the uterine wall and into the amniotic cavity. Discard the first 1-2ml of amniotic fluid to minimize the possibility of maternal cell contamination, and dispense 15-30 ml of the remaining fluid into two or three sterile 15 ml centrifuge tubes.
Contact the laboratory(608-262-0402) to obtain Amniotic Fluid kits.
Kits include: 2-15ml sterile conical tubes, 1 Test requisition form (#131), and 1 Biohazard bag and absorbent pad.
Unacceptable Conditions: Sample must not be frozen.
Requisition Form: Cytogenetics Lab Genetic Diagnosis Form #131
Required Information: Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, gestational age, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results Include: Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results.
Limitations: The cytogenetic methods used in this analysis do not routinely detect small structural rearrangements, microdeletions, or low level (<38%) mosaicism. Viable cells may be maternally derived.
Additional Tests Recommended: Ordered only in conjunction with Illumina Microarray Analysis
Additional Comments:
Methodology: Microscopic analysis of G-banded chromosomes.
Includes: G-banded chromosome analysis of cultured amniocytes. Includes in situ culture of cells from amniotic fluid, examination of 5 metaphase cells from 5 independent colonies, and preparation of 1 karyogram.
CPT Code: 88235, 88261
Price: For pricing information, please call 608-262-0402.
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