Chromosome Analysis, Amniotic Fluid-Abridged, for Pre-natal Genetic Diagnosis
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| WSLH Department: | Cytogenetics |
| WSLH Test Code: | 852 |
| Day(s) Performed: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | Approximately 7-14 days, with an average of 11 days (longer for specimens with small volume, containing blood or slow growth). (Reports are issued Monday-Friday 7:45 AM - 4:30 PM) |
| Recommended Uses: | Abridged chromosome analysis to be ordered in conjunction with Prenatal Microarray for a fetus with one or more major structural abnormalities identified on ultrasonographic examination. |
| Contraindications: | |
| Additional Tests Performed: |
| Patient Preparations: | |
| Specimen Requirements: | 15-30 ml amniotic fluid (will be used for both the Abridged chromosome analysis and the Prenatal Microarray) |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 to 48 hours of collection. |
| Collection Kit/Container: | |
| Collection Instructions: | Collect the fluid under sterile, ultrasound guided conditions using a 22-gauge needle inserted through the uterine wall and into the amniotic cavity. Discard the first 1-2ml of amniotic fluid to minimize the possibility of maternal cell contamination, and dispense 15-30 ml of the remaining fluid into two or three sterile 15 ml centrifuge tubes. Kits include: 2-15ml sterile conical tubes, 1 Test requisition form (#131), and 1 Biohazard bag and absorbent pad. Contact the laboratory(608-262-0402) to obtain Amniotic Fluid kits. |
| Unacceptable Conditions: | Sample must not be frozen. |
| Requisition Form: |
Cytogenetics Lab Genetic Diagnosis Form #131
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, gestational age, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results. |
| Limitations: | The cytogenetic methods used in this analysis do not routinely detect small structural rearrangements, microdeletions, or low level (<38%) mosaicism. Viable cells may be maternally derived. |
| Additional Tests Recommended: | Ordered only in conjunction with Illumina Microarray Analysis |
| Additional Comments: |
| Methodology: | Microscopic analysis of G-banded chromosomes. |
| Includes: | G-banded chromosome analysis of cultured amniocytes. Includes in situ culture of cells from amniotic fluid, examination of 5 metaphase cells from 5 independent colonies, and preparation of 1 karyogram. |
| CPT Code: | 88235, 88261 |
| Price: | For pricing information, please call 608-262-0402. |