Bordetella pertussis/parapertussis PCR (also detects B. holmesii)
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP00311 |
| Day(s) Performed: | 3 times weekly - Monday, Wednesday, Friday |
| Turn-around Time: | 1-3 days |
| Recommended Uses: | Test of choice for the diagnosis of Bordetella pertussis (whooping cough) and Bordetella parapertussis. |
| Pre-approval: | N |
| Contraindications: | |
| Additional Tests Performed: |
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| Preparation for Collection: | |
| Specimen Requirements: | Dry nasopharyngeal swabs, or nasopharyngeal aspirates. |
| Collection Instructions: | Kit 30; Dacron Swab or NP aspirate submitted in sterile, dry tube. |
| Collection Kit/Container: |
Pertussis (a.k.a Whooping Cough, Bordetella)
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| Requisition Form: |
CDD Requisition Form A
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Rejection Criteria: | Specimens should NOT be submitted in charcoal-based media or on wooden shaft swabs. Do NOT use cotton swabs. |
| Specimen Handling: | Specimens should be transported to the laboratory with cool pack. |
| Packaging Requirements: | |
| Courier Information: | |
| Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Results Include: | B. pertussis, B. parapertussis or B. holmesii DNA detected/ not detected |
| Limitations: | This test is not FDA cleared or approved. Test results must be correlated with patient history and clinical presentation to confirm as a case of B. pertussis or B. parapertussis infection. A negative result does not rule out infection with B. pertussis or B. parapertussis. B. holmesii is an uncommon cause of respiratory illness in humans. |
| Additional Tests Recommended: | Please include an additional swab in appropriate transport medium for Bordetella culture to use for public health/epidemiologic purposes. Cultures will be performed on PCR positive specimens to obtain an isolate for characterization. There will be no charge for this testing and culture results will not be reported to the submitter. See test code MP00623 for collection information. |
| Additional Comments: |
| Methodology: | PCR |
| Includes: | PCR assay for the detection of Bordetella pertussis, Bordetella parapertussis and Bordetella holmseii DNA in respiratory specimens. |
| Regulatory Compliance: |
| CPT Code: | 87798x3 |
| Price: | $175.00 Price Effective 1/1/25 |
| Fee Exempt Eligible: | N |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |