TCR Rearrangement (TRA/D), FISH
| WSLH Department: | Cytogenetics |
| WSLH Test Code: | 882F91 |
| Availability: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | Approximately 4-6 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM) |
| Recommended Uses: | Detection of rearrangements of the TCR gene associated with T-cell lymphoma and prolymphocytic leukemia. Aids in diagnosis and prognosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant. May be performed in conjunction with other FISH assays, chromosome analysis (for bone marrow, blood, and fresh tissue only) or as an independent test. |
| Contraindications: | |
| Additional Tests Performed: |
| Patient Preparations: | |
| Specimen Requirements: | Bone marrow: 0.5-1.0 ml early aspirate bone marrow collected in sodium heparin OR Blood: 1-2 ml whole blood collected in sodium heparin vacuum type tube OR Fresh tissue: At least 0.3cm cubed section of tumor; avoid areas of necrosis |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 hours of collection. |
| Collection Kit/Container: | |
| Collection Instructions: | ** Bone marrow: 0.5-1.0 ml bone marrow from the 1st or 2nd aspirate. Material submitted for cytogenetic analysis must be from an early aspirate to avoid dilution with blood. Rinse the syringe to be used for aspiration by drawing sodium heparin solution (1000 USP units/ml) into the syringe and then expelling all the heparin solution (over-heparinization is toxic to the cells). Transfer the marrow to a sterile, sodium heparin vacuum type tube for delivery to State Lab. If an aspirate is unsuccessful, a 10mm bone core biopsy may be submitted in a sterile transport medium with sodium heparin. ** Blood: Draw blood using aseptic techniques into a sterile sodium heparin vacuum type tube. Invert tube to mix. If using larger tubes, draw full tube of blood to avoid over-treatment with anti-coagulant. ** Fresh tissue: A 0.3-1.0cm cubed section collected using aseptic procedures; avoid areas of necrosis. Place the specimen in a sterile tissue vial containing transport media. |
| Unacceptable Conditions: | Bone marrow/blood that is clotted or hemolyzed is not acceptable. Samples must not be frozen. Plasma and serum are not acceptable. |
| Requisition Form: |
Cytogenetics Lab Neoplasia Diagnosis Form #132
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Number of nuclei examined, percent positive for rearrangement, and the reference range for false positives based on the average number of cells with an abnormal signal pattern in two hundred control cells. Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results. |
| Limitations: | Detects rearrangements involving this gene; the translocation partner chromosome cannot be determined by this test. Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen. |
| Additional Tests Recommended: | |
| Additional Comments: | If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen. |
| Methodology: | Fluorescence in situ hybridization performed on interphase nuclei using a break-apart DNA probe (LSI TCR dual color, break apart rearrangement; Vysis, Inc) specific for the TCR gene in 14q11.2. This probe is designed to detect rearrangements of the TCR gene including translocations and deletions. Translocation partner chromosomes cannot be determined by this test. |
| Includes: | FISH analysis of 200 interphase cells for rearrangement of the T-cell receptor gene (TCR) alpha and delta region. |
| CPT Code: | 88271x2, 88275 |
| Price: | For pricing information, please call 608-262-0402. |