FIP1L1-CHIC2-PDGFRA Rearrangement, FISH Print

WSLH Department: Cytogenetics
WSLH Test Code: 882F89
Day(s) Performed: Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time: Approximately 4-6 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses: Detection of rearrangements of the FIP1L1-CHIC-2-PDGFRA region associated with myeloproliferative neoplasms with eosinophilia. Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant. May be performed in conjunction with other FISH assays, chromosome analysis (for bone marrow, blood, and fresh tissue only) or as an independent test.
Contraindications:
Additional Tests Performed:
Patient Preparations:
Specimen Requirements: 0.5-1.0 ml early aspirate bone marrow collected in sodium heparin
OR
1-2 ml whole blood collected in sodium heparin vacuum type tube
Specimen Handling & Transport: Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 to 48 hours of collection.
Collection Kit/Container:
Collection Instructions: **Bone marrow: 0.5-1.0ml bone marrow from 1st or 2nd aspirate. Material submitted for cytogenetic analysis must be from an early aspirate to avoid dilution with blood. Draw sodium heparin solution (1000 USP units/ml) into the syringe to be used for aspiration and then expel (over-heparinization is toxic to the cells). Transfer the marrow to a sterile, Na-Heparin vacuum type tube for delivery to State Lab. If an aspirate is unsuccessful, a 10mm bone core biopsy may be submitted in a sterile transport medium with Na-Heparin.

**Blood: Draw blood using aseptic techniques into a sterile Na-Heparin vacuum type tube. Invert tube to mix. If using larger tubes, draw to full volume to avoid over-treatment with heparin.
Unacceptable Conditions: Bone marrow/blood that is clotted or hemolyzed is not acceptable. Samples must not be frozen. Plasma and serum are not acceptable.
Requisition Form: Cytogenetics Lab Neoplasia Diagnosis Form #132
Required Information: Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results Include: Number of nuclei examined, percent positive for rearrangement of FIP1L1-CHIC2-PDGFRA region, and the reference range for false positives based on the number of cells with an abnormal signal pattern in 200 control cells. Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results.
Limitations: Detects rearrangements involving this region; the translocation partner chromosome cannot be determined by this test. Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen.
Additional Tests Recommended:
Additional Comments: If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen.
Methodology: Fluorescence in situ hybridization performed on interphase nuclei using a DNA probe (FIP-CHIC2-PDGFRA tri color, rearrangement; CytoTest) specific for the FIP1L1-CHIC2-PDGFRA region in 4q12. This probe is designed to detect rearrangements of the FIP1L1-CHIC2-PDGFRA region including translocations and deletions of CHIC2. Translocation partner chromosomes cannot be determined by this test.
Includes: FISH analysis of 200 cells for rearrangement of the FIP1L1-CHIC2-PDGFRA region
CPT Code: 88271x3, 88275
Price: For pricing information, please call 608-262-0402.
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