BCL6 Rearrangement, FISH
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| WSLH Department: | Cytogenetics |
| WSLH Test Code: | 882F88 |
| Day(s) Performed: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | Approximately 4-6 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM) |
| Recommended Uses: | Detection of rearrangements of the BCL6 gene associated with B-cell non-Hodgkin Lymphoma (NHL). Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant. May be performed in conjunction with other FISH assays, chromosome analysis (for bone marrow, blood, and fresh tissue only) or as an independent test. |
| Contraindications: | |
| Additional Tests Performed: |
| Patient Preparations: | |
| Specimen Requirements: | 0.5-1.0 ml early aspirate bone marrow collected in sodium heparin OR 1-2 ml whole blood collected in sodium heparin vacuum type tube OR Fresh tissue: At least 0.3cm cubed section of tumor; avoid areas of necrosis. OR Paraffin embedded tissue: Five 4-micron sections on immunohistochemistry slides, the middle section stained with H&E. |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 to 48 hours of collection. |
| Collection Kit/Container: | |
| Collection Instructions: | **Bone marrow: 0.5-1.0ml bone marrow from 1st or 2nd aspirate. Material submitted for cytogenetic analysis must be from an early aspirate to avoid dilution with blood. Draw sodium heparin solution (1000 USP units/ml) into the syringe to be used for aspiration and then expel (over-heparinization is toxic to the cells). Transfer the marrow to a sterile, Na-Heparin vacuum type tube for delivery to State Lab. If an aspirate is unsuccessful, a 10mm bone core biopsy may be submitted in a sterile transport medium with Na-Heparin. **Blood: Draw blood using aseptic techniques into a sterile Na-Heparin vacuum type tube. Invert tube to mix. If using larger tubes, draw to full volume to avoid over-treatment with heparin. **Fresh tissue: A 0.3-1.0cm cubed section collected using aseptic procedures; avoid areas of necrosis. Place the specimen in a sterile tissue vial containing transport media. **FFPE tissue: Tissue specimen should be fixed in 10% neutral buffered formalin for 6-48 hours. |
| Unacceptable Conditions: | Bone marrow/blood that is clotted or hemolyzed is not acceptable. Samples must not be frozen. Plasma and serum are not acceptable. FFPE tissue specimen must not be de-calcified. |
| Requisition Form: |
Cytogenetics Lab Neoplasia Diagnosis Form #132
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Number of nuclei examined, percent positive for BCL6 rearrangement, and the reference range for false positives based on the number of cells with an abnormal signal pattern in 200 control cells. Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results. |
| Limitations: | Detects rearrangements involving this gene; the translocation partner chromosome cannot be determined by this test. Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen. This probe will not detect small deletions or duplications or base pair changes. |
| Additional Tests Recommended: | |
| Additional Comments: | If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen. |
| Methodology: | Fluorescence in situ hybridization performed on interphase nuclei using a break-apart DNA probe (LSI BCL6 dual color, break apart rearrangement; Abbott Molecular) specific for the BCL6 gene in 3q27. This probe is designed to detect rearrangements of the BCL6 gene including translocations and deletions. Translocation partner chromosomes cannot be determined by this test. |
| Includes: | FISH analysis of 200 interphase cells for rearrangement of the BCL6 gene |
| CPT Code: | 88271x2, 88275 |
| Price: | For pricing information, please call 608-262-0402. |