Chromosome Analysis, Unstimulated Blood, for Hematologic Disorders
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| WSLH Department: | Cytogenetics |
| WSLH Test Code: | 811 |
| Day(s) Performed: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | Approximately 7-10 days, RUSH specimens reported by phone or fax in 3-4 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM) |
| Recommended Uses: | Identification of chromosome abnormalities associated with hematologic disorders such as Acute Myeloid Leukemia (AML) and Acute Lymphoid Leukemia (ALL). |
| Contraindications: | This test is not for the identification of chromosome abnormalities associated with constitutional genetic disorders. |
| Additional Tests Performed: |
| Patient Preparations: | |
| Specimen Requirements: | 1.5-2.5 ml whole blood collected in sodium heparin. |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 to 48 hours of collection. |
| Collection Kit/Container: | |
| Collection Instructions: | Draw blood using aseptic techniques into a sterile sodium heparin vacuum type tube. Invert tube to mix. If using larger tubes, draw to full volume to avoid over-treatment with heparin. |
| Unacceptable Conditions: | Blood that is collected in EDTA, sodium citrate, or other anti-coagulants is not acceptable. Blood that is clotted or hemolyzed is not acceptable. Blood must not be frozen. Plasma and serum are not acceptable. |
| Requisition Form: |
Cytogenetics Lab Neoplasia Diagnosis Form #132
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results. |
| Limitations: | Analysis is dependent on the presence of actively dividing cells in the blood specimen. The absence of an abnormal clone in unstimulated blood cells does not exclude chromosome abnormalities in the bone marrow. |
| Additional Tests Recommended: | Fluorescence in situ hybridization analysis may be recommended to characterize chromosome abnormalities; if performed there will be an additional charge. |
| Additional Comments: |
| Methodology: | Microscopic analysis of G-banded chromosomes. |
| Includes: | G-banded chromosome analysis of unstimulated, spontaneously dividing, peripheral blood cells. Includes short term culture of blood cells, examination of at least 20 metaphase cells (when possible), and preparation of two karyograms. Additional cells may be examined if indicated by initial results. Additional karyograms may be prepared if multiple cell lines are observed. Quality and number of cells analyzed may be limited if specimen is not adequate. |
| CPT Code: | 88237, 88264 |
| Price: | For pricing information, please call 608-262-0402. |