Deletion 1p, FISH, Glial neoplasia Print
WSLH Department: | Cytogenetics |
WSLH Test Code: | 886F5 |
Availability: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
Turn-around Time: | Approximately 5-8 days, with an average of 5 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM) |
Recommended Uses: | Detection of relative loss of the short arm of chromosome 1. Aids in diagnosis of glial neoplasia including astrocytoma and oligodendroglioma. |
Contraindications: | |
Additional Tests Performed: |
Patient Preparations: | |
Specimen Requirements: | Paraffin embedded tissue: Five 4-micron sections on immunohistochemistry slides, the middle section stained with H&E. |
Specimen Handling & Transport: | Store and transport specimens at room temperature. |
Collection Kit/Container: | |
Collection Instructions: | FFPE tissue: Tissue specimen should be fixed in 10% neutral buffered formalin for 6-72 hours. |
Unacceptable Conditions: | FFPE tissue specimen must not be de-calcified. |
Requisition Form: |
Cytogenetics Lab Neoplasia Diagnosis Form #132
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Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
Results Include: | Ratio of 1p36 to 1q25 signals. Deletion 1p detected/not detected |
Limitations: | |
Additional Tests Recommended: | Test 886F6 for detection of deletion of 19q. |
Additional Comments: |
Methodology: | Fluorescence in situ hybridization performed on interphase nuclei from paraffin embedded tissue using DNA probes (LSI 1p36/LSI 1q25; Abbott Molecular) covering the region including the TP73 and EGFL3 genes in 1p36 and probes covering the region including the ABL2 and ANGPTL1 genes in 1q25 as an internal control |
Includes: | FISH analysis of 200 cells for copy number of chromosome 1p compared to copy number of chromosome 1q |
CPT Code: | 88271x2, 88275 |
Price: | For pricing information, please call 608-262-0402. |