Deletion 1p, FISH, Glial neoplasia Print

WSLH Department: Cytogenetics
WSLH Test Code: 886F5
Availability: Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time: Approximately 5-8 days, with an average of 5 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses: Detection of relative loss of the short arm of chromosome 1. Aids in diagnosis of glial neoplasia including astrocytoma and oligodendroglioma.
Contraindications:
Additional Tests Performed:
Patient Preparations:
Specimen Requirements: Paraffin embedded tissue: Five 4-micron sections on immunohistochemistry slides, the middle section stained with H&E.
Specimen Handling & Transport: Store and transport specimens at room temperature.
Collection Kit/Container:
Collection Instructions: FFPE tissue: Tissue specimen should be fixed in 10% neutral buffered formalin for 6-72 hours.
Unacceptable Conditions: FFPE tissue specimen must not be de-calcified.
Requisition Form: Cytogenetics Lab Neoplasia Diagnosis Form #132
Required Information: Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results Include: Ratio of 1p36 to 1q25 signals. Deletion 1p detected/not detected
Limitations:
Additional Tests Recommended: Test 886F6 for detection of deletion of 19q.
Additional Comments:
Methodology: Fluorescence in situ hybridization performed on interphase nuclei from paraffin embedded tissue using DNA probes (LSI 1p36/LSI 1q25; Abbott Molecular) covering the region including the TP73 and EGFL3 genes in 1p36 and probes covering the region including the ABL2 and ANGPTL1 genes in 1q25 as an internal control
Includes: FISH analysis of 200 cells for copy number of chromosome 1p compared to copy number of chromosome 1q
CPT Code: 88271x2, 88275
Price: For pricing information, please call 608-262-0402.
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