Patient Preparations: |
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Specimen Requirements: |
Bone marrow: 0.5-1.0 ml early aspirate bone marrow collected in sodium heparin OR Blood: 1-2 ml whole blood collected in sodium heparin vacuum type tube OR Fresh tissue: At least 0.3cm cubed section of tumor; avoid areas of necrosis Paraffin embedded tissue (FFPE): Five 4-micron sections on immunohistochemistry slides, the middle section stained with H&E |
Specimen Handling & Transport: |
Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F ). DO NOT FREEZE. The laboratory must receive specimens within 24 hours of collection. |
Collection Kit/Container: |
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Collection Instructions: |
** Bone marrow: 0.5-1.0 ml bone marrow from the 1st or 2nd aspirate. Material submitted for cytogenetic analysis must be from an early aspirate to avoid dilution with blood. Rinse the syringe to be used for aspiration by drawing sodium heparin solution (1000 USP units/ml) into the syringe and then expelling all the heparin solution (over-heparinization is toxic to the cells). Transfer the marrow to a sterile, sodium heparin vacuum type tube for delivery to State Lab. If an aspirate is unsuccessful, a 10mm bone core biopsy may be submitted in a sterile transport medium with sodium heparin. ** Blood: Draw blood using aseptic techniques into a sterile sodium heparin vacuum type tube. Invert tube to mix. If using larger tubes, draw full tube of blood to avoid over-treatment with anti-coagulant. ** Fresh tissue: A 0.3-1.0cm cubed section collected using aseptic procedures; avoid areas of necrosis. Place the specimen in a sterile tissue vial containing transport media. ** FFPE tissue: Tissue specimen should be fixed in 10% neutral buffered formalin for 6-48 hours. |
Unacceptable Conditions: |
Bone marrow/blood that is clotted or hemolyzed is not acceptable. Samples must not be frozen. Plasma and serum are not acceptable. FFPE tissue specimen must not be de-calcified. |
Requisition Form: |
Cytogenetics Lab Neoplasia Diagnosis Form #132
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Required Information: |
Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |