EWSR1 Rearrangement, FISH
| WSLH Department: | Cytogenetics |
| WSLH Test Code: | 882F81 |
| Availability: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | Approximately 4-6 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM) |
| Recommended Uses: | Detection of rearrangements of the EWSR1 gene associated with Ewing sarcoma. Aids in diagnosis of malignancy and for monitoring disease after treatment. May be performed in conjunction with other FISH assays, chromosome analysis (for fresh tissue only) or as an independent test. |
| Contraindications: | |
| Additional Tests Performed: |
| Patient Preparations: | |
| Specimen Requirements: | Fresh tissue: At least 0.3cm cubed section of tumor; avoid areas of necrosis. OR Paraffin embedded tissue: Five 4-micron sections on immunohistochemistry slides, the middle section stained with H & E. |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 hours of collection. |
| Collection Kit/Container: | |
| Collection Instructions: | **Fresh tissue: A 0.3-1.0cm cubed section collected using aseptic procedures; avoid areas of necrosis. Place the specimen in a sterile tissue vial containing transport media. **FFPE tissue: Tissue specimen should be fixed in 10% neutral buffered formalin for 6-48 hours. |
| Unacceptable Conditions: | Samples must not be frozen. FFPE tissue specimen must not be de-calcified. |
| Requisition Form: |
Cytogenetics Lab Neoplasia Diagnosis Form #132
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Number of nuclei examined, percent positive for rearrangement of EWSR1 gene, and the reference range for false positives based on the number of cells with an abnormal signal pattern in 200 control cells. Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results. |
| Limitations: | Detects rearrangements involving this gene; the translocation partner chromosome cannot be determined by this test. Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen. |
| Additional Tests Recommended: | |
| Additional Comments: | If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen. |
| Methodology: | Fluorescence in situ hybridization performed on interphase nuclei using a break-apart DNA probe (LSI EWSR1 dual color, rearrangement; Vysis, Inc) specific for the EWSR gene in 22q12. This probe is designed to detect rearrangements of the EWSR1 gene including translocations and deletions. Translocation partner chromosomes cannot be determined by this test. |
| Includes: | FISH analysis of 200 cells for rearrangement of the EWSR gene |
| CPT Code: | 88271x2, 88275 |
| Price: | For pricing information, please call 608-262-0402. |