Stillbirth Aneuploidy Panel, FISH, Paraffin embedded fetal tissue
| WSLH Department: | Cytogenetics |
| WSLH Test Code: | 875 |
| Availability: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | Approximately 10-14 days, with an average of 9 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM) |
| Recommended Uses: | Stillbirth or spontaneous miscarriages where chromosome analysis is unsuccessful. |
| Contraindications: | |
| Additional Tests Performed: |
| Patient Preparations: | |
| Specimen Requirements: | Paraffin embedded tissue (FFPE): Seven 4-micron sections on immunohistochemistry slides, the middle section stained with H&E |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 hours of collection. |
| Collection Kit/Container: | |
| Collection Instructions: | FFPE tissue: Tissue specimen should be fixed in 10% neutral buffered formalin for 6-48 hours. |
| Unacceptable Conditions: | Necrotic tissue may not contain nuclei with intact DNA. |
| Requisition Form: |
Cytogenetics Lab Genetic Diagnosis Form #131
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, gestational age, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Evidence/no evidence of trisomy 13, 18, and/or 21. Sex chromosome signal pattern. |
| Limitations: | FISH results reflect copy number only for the loci of the probes used, and do not rule out isochromosomes or structural abnormalities of chromosomes 13, 18, 21, X, and Y. |
| Additional Tests Recommended: | |
| Additional Comments: |
| Methodology: | Fluorescence in situ hybridization (FISH) analysis on interphase nuclei (nuc ish) is performed on paraffin embedded placenta cells using a DNA probe set (AneuVysion: CEP 18/CEP X/CEP Y, LSI 13/21; CEP 16, LSI BCR, Abbott molecular, Inc). |
| Includes: | FISH analysis of paraffin embedded placenta or fetal tissue to determine copy number of chromosomes 13, 16, 18, 21, 22, X and Y. |
| CPT Code: | 88271x7, 88275 |
| Price: | For pricing information, please call 608-262-0402. |