| Patient Preparations: |
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| Specimen Requirements: |
2-5ml amniotic fluid (in addition to specimen collected for chromosome analysis) |
| Specimen Handling & Transport: |
Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F ). DO NOT FREEZE. The laboratory must receive specimens within 24 to 48 hours of collection. |
| Collection Kit/Container: |
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| Collection Instructions: |
Kits include: 2-15ml sterile conical tubes, 1 Test requisition form (#131), and 1 Biohazard bag and absorbent pad. Contact the laboratory (608-262-0402) to obtain Amniotic Fluid kits.
Collect amniotic fluid under sterile, ultrasound guided conditions using a 22-gauge needle inserted through the uterine wall and into the amniotic cavity. Discard the first 1-2ml of amniotic fluid to minimize the possibility of maternal cell contamination. Dispense amniotic fluid into a sterile 15 ml centrifuge tube. |
| Unacceptable Conditions: |
Specimens contaminated with maternal blood are not appropriate since maternal blood cells in the specimen may not be distinguishable from female fetal cells by this test.
Sample must not be frozen. |
| Requisition Form: |
Cytogenetics Lab Genetic Diagnosis Form #131
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| Required Information: |
Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, gestational age, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
