Chromosome Analysis, Tumor/Pleural Effusion/Ascites fluid, for Neoplastic Disorders
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| WSLH Department: | Cytogenetics |
| WSLH Test Code: | 836 |
| Day(s) Performed: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | Approximately 6-14 days, with an average of 9 days. (For tumors that grow as monolayers additional time for culturing may be required.) (Reports are issued Monday-Friday 7:45 AM - 4:30 PM) |
| Recommended Uses: | Identification of chromosome abnormalities associated with solid tumors and neoplasias. |
| Contraindications: | |
| Additional Tests Performed: |
| Patient Preparations: | |
| Specimen Requirements: | Tumor sample at least 0.3 cm cubed; 10-20ml of pleural effusion or ascites fluid |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 to 48 hours of collection. |
| Collection Kit/Container: | |
| Collection Instructions: | **A 0.3-1.0cm cubed section should be collected using aseptic procedures; avoid areas of necrosis. Place the specimen in a sterile tissue vial containing transport media. **Collect the fluid under sterile conditions. Dispense 10-20 ml of the fluid into two sterile 15 ml centrifuge tubes or a sterile specimen cup. |
| Unacceptable Conditions: | Samples must not be frozen or processed with formalin. |
| Requisition Form: |
Cytogenetics Lab Neoplasia Diagnosis Form #132
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results. |
| Limitations: | Analysis is dependent on the presence of actively dividing tumor cells in the specimen. |
| Additional Tests Recommended: | Fluorescence in situ hybridization analysis may be recommended to characterize chromosome abnormalities; if performed there will be an additional charge. |
| Additional Comments: |
| Methodology: | Microscopic analysis of G-banded chromosomes. |
| Includes: | G-banded chromosome analysis of unstimulated, spontaneously dividing, tumor cells. Includes short term culture of cells from tumor or effusion, examination of at least 20 metaphase cells (when possible), and preparation of two karyograms. Additional cells may be examined if indicated by initial results. Additional karyograms may be prepared if multiple cell lines are observed. Quality and number of cells analyzed may be limited if specimen is not adequate. |
| CPT Code: | 88239, 88264, 88280 |
| Price: | For pricing information, please call 608-262-0402. |