Chromosome Analysis, Bone Marrow, for Hematologic Disorders Print

WSLH Department: Cytogenetics
WSLH Test Code: 812
Availability: Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time: Approximately 7-10 days, RUSH specimens reported by phone or fax in 3-5 days. (Reports are issued Monday-Friday, 7:45 AM - 4:30 PM)
Recommended Uses: Identification of chromosome abnormalities associated with hematologic disorders such as Acute Myeloid Leukemia (AML), Acute Lymphoid Leukemia (ALL), lymphoma, myeloma. Aids in diagnosis and follow-up after treatment or bone marrow transplant.
Additional Tests Performed:
Patient Preparations:
Specimen Requirements: 1-2 ml early aspirate of bone marrow collected in sodium heparin.
Specimen Handling & Transport: Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 hours of collection.
Collection Kit/Container:
Collection Instructions: 1.0-2.0ml bone marrow from 1st or 2nd aspirate. Material submitted for cytogenetic analysis must be from an early aspirate to avoid dilution with blood. Draw sodium heparin solution (1000 USP units/ml) into the syringe to be used for aspiration and then expel (over-heparinization is toxic to the cells). Transfer the marrow to a sterile, Na-Heparin vacuum type tube for delivery to State Lab. If an aspirate is unsuccessful, a 10mm bone core biopsy may be submitted in a sterile transport medium with Na-Heparin.
Unacceptable Conditions: Bone marrow that is collected in EDTA, sodium citrate, or other anti-coagulants is not acceptable. Bone marrow that is clotted or hemolyzed is not acceptable. Bone marrow must not be frozen.
Requisition Form: Cytogenetics Lab Neoplasia Diagnosis Form #132
Required Information: Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results Include: Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results.
Limitations: Analysis is dependent on the presence of actively dividing cells in the bone marrow specimen.
Additional Tests Recommended: Fluorescence in situ hybridization analysis may be recommended to characterize chromosome abnormalities; if performed there will be an additional charge.
Additional Comments:
Methodology: Microscopic analysis of G-banded chromosomes.
Includes: G-banded chromosome analysis of unstimulated, spontaneously dividing, bone marrow cells. Includes short term culture of bone marrow, examination of at least 20 metaphase cells (when possible), and preparation of two karyograms. Additional cells may be examined if indicated by initial results. Additional karyograms may be prepared if multiple cell lines are observed. Quality and number of cells analyzed may be limited if specimen is not adequate.
CPT Code: 88237, 88264
Price: For pricing information, please call 608-262-0402.
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