Brucella Culture
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP00350 |
| Day(s) Performed: | Daily, Monday - Saturday |
| Turn-around Time: | 3 days - 4 weeks |
| Recommended Uses: | To detect infections caused by Brucella species. |
| Pre-approval: | N |
| Contraindications: | Patients with no travel history or contact to animal sources (including unpasteurized dairy) have a low likelihood of Brucella infection. |
| Additional Tests Performed: |
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| Preparation for Collection: | |
| Specimen Requirements: | Blood and/or bone marrow in commercial aerobic blood culture bottle with CO2; Sterile vial for fluid specimens other than blood and bone marrow; Sterile vial with non-bacteriostatic saline for tissues |
| Collection Instructions: | Aerobic blood culture as per users protocol. |
| Collection Kit/Container: |
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| Requisition Form: |
CDD Requisition Form A
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| Required Information: | |
| Rejection Criteria: | Formalinized tissue is unacceptable for culture. |
| Specimen Handling: | Refrigerator temperature for specimens other than blood culture bottles. Ship blood culture bottles at ambient temperatures. It is recommended that specimens are received in the laboratory within 24-48 hours of collection. |
| Packaging Requirements: | |
| Courier Information: | |
| Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Results Include: | Negative for Brucella species or Culture shows Brucella (with species identification) |
| Limitations: | Blood cultures are only useful early in the acute phase of the disease. |
| Additional Tests Recommended: | See Brucella Antibody (test #SS02002) |
| Additional Comments: | If Brucella is suspected, please call the WSLH prior to submission of clinical specimens. It is best that blood cultures be incubated at the clinical laboratory. If positive for an isolate that cannot be ruled out as Brucella, submit the isolate to WSLH and order test code MP00470. Culture identification will be automatically confirmed by PCR. Extreme caution should be taken when handling isolates or specimens suspected of containing Brucella species. Brucella is a Select Agent under Federal Regulations and must be reported to the CDC Select Agent Program. |
| Methodology: | Bacterial culture, PCR |
| Includes: | Blood, bone marrow, abscess or tissue for isolation and identification of Brucella species. |
| Regulatory Compliance: |
| CPT Code: | 87081 |
| Price: | $61.00 Price Effective 1/1/25 |
| Fee Exempt Eligible: | N |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |