Shiga toxin PCR Print

WSLH Department: CDD - Communicable Disease Division
WSLH Test Code: MP00545
Availability: Weekly
Turn-around Time: 1-7 days
Recommended Uses: 1. Detection of Shiga toxin-producing bacteria in stool specimens from cases of community-acquired diarrhea and cases with suspected or confirmed hemolytic uremic syndrome (HUS).
2. Confirmation of Shiga toxin EIAs or other other testing method (i.e. molecular syndromic panels).
Additional Tests Performed:
Patient Preparations:
Specimen Requirements: • Stool specimens in Cary Blair transport media
• Bacterial isolates
• Enrichment broth (e.g. GN or MacConkey broth)
Specimen Handling & Transport: • Store stools at 2-8 degrees Celsius and transport with cool pack.
• After incubation, store enrichment broths at 2-8 degrees Celsius C and transport with cool pack.
• Isolates may be stored and transported at ambient temperatures
Collection Kit/Container: Stool Culture (Enteric Pathogen Culture)
Collection Instructions: Collect stool specimens according to instructions in Kit #10. Refer to instructions used at your facility for proper inoculation and handling of enrichment broths.
Unacceptable Conditions: Unpreserved stool specimen
Requisition Form: CDD Requisition Form A
Required Information: Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results Include: Shiga toxin 1 DNA detected/ not detected;
Shiga toxin 2 DNA detected/ not detected;
eae DNA detected/not detected;
ehxA DNA detected/not detected
Additional Tests Recommended: Routine stool culture for enteric bacterial pathogens, including E. coli O157
Additional Comments:
Methodology: Real-time PCR
Includes: Real-time PCR for the detection of Shiga toxin 1 and Shiga toxin 2 genetic markers from cultures of stool specimens.
CPT Code: 87798
Price: $450.00
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