Mycobacterium tuberculosis Susceptibility First Line Drugs
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MM00204 |
| Day(s) Performed: | Monday - Friday |
| Turn-around Time: | 3-6 weeks |
| Recommended Uses: | To determine the in vitro susceptibility of M. tuberculosis complex organisms to first line antimicrobial agents. |
| Pre-approval: | No |
| Contraindications: | |
| Additional Tests Performed: |
GeneXpert MTB/RIF (MM00261) will automatically be performed on all isolates submitted for 1st-line DST (fee-exempt). Isolates resistant to rifampin or two or more first-line drugs are automatically referred to CDC for second-line drug susceptibility testing (fee-exempt). The first M. tuberculosis complex isolate per patient is automatically genotyped as part of the CDC universal genotyping program (fee-exempt). |
| Preparation for Collection: | |
| Specimen Requirements: | M. tuberculosis complex isolate in broth or solid media |
| Collection Instructions: | |
| Collection Kit/Container: |
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| Requisition Form: |
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| Required Information: | Identification of organism as a member of the M. tuberculosis complex. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers. |
| Rejection Criteria: | |
| Specimen Handling: | Cultures may be transported to the laboratory at ambient temperatures. Avoid temperature extremes during transport. |
| Packaging Requirements: | |
| Courier Information: | Contact Purple Mountain Dispatch at 800-990-9668 for specimen pickup |
| Specimen Receiving Hours: | Monday-Friday 6:00 AM - 4:30 PM, Saturday 6:30 AM - 12:30 PM |
| Results Include: | M. tuberculosis complex organisms Susceptible, Resistant, or Indeterminate to the listed antimicrobial agents using criteria established by the Clinical and Laboratory Standards Institute (CLSI). |
| Limitations: | Test can only be performed on pure M. tuberculosis complex isolates. Mixed or contaminated cultures will extend turnaround time significantly and may prevent susceptibility testing if pure M. tuberculosis complex cannot be isolated. |
| Additional Tests Recommended: | |
| Additional Comments: |
| Methodology: | MGIT broth method |
| Includes: | Susceptibility tests of M. tuberculosis complex organisms with two concentrations of Isoniazid (I), one concentration of Ethambutol (E), Rifampin (R) and Pyrazinamide (PZA). |
| Regulatory Compliance: |
| CPT Code: | 87188x5 |
| Price: | $271.00 |
| Fee Exempt Eligible: | No |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |