HIV-1 Proviral DNA PCR Print

WSLH Department: CDD - Communicable Disease Division
WSLH Test Code: SS00091
Day(s) Performed:

Daily, Monday - Friday
Turn-around Time:

1 Day
Recommended Uses:

Approved for testing neonates whose mothers are HIV antibody positive or considered to be at high risk for HIV infection; patients with HIV Ag/Ab Combo positive and with negative or indeterminate Geenius results for HIV; patients suspected of acute HIV infection prior to seroconversion.
Pre-approval:

Please call the lab prior to submission to arrange for testing (800-862-1013).
Contraindications:

Not approved as a primary screening assay (except for neonates) 
Additional Tests Performed:
Preparation for Collection:
Specimen Requirements:

One 7 ml EDTA (lavender top) vacutainer of whole blood for adults. Greater than or equal to 400 ul EDTA (lavender top) vacutainer of whole blood for pediatrics. Store refrigerated at 2-8°C. Patient specimen must be received within 5 days of collection.
Collection Instructions:

Routine blood draw

Comply with Wisconsin informed opt-out statutes.
Collection Kit/Container:
Requisition Form:
Required Information:

Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Rejection Criteria:

Specimen collected in container other than EDTA or ACD vacutainer tube. Specimens received >5 days after collection.
Specimen Handling:

Patient specimen must be received within 5 days of collection. Ship on frozen cold pack.
Packaging Requirements:
Courier Information:

This test is not eligible for fee-exempt courier service.
Specimen Receiving Hours:

Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Results Include:

HIV-1 proviral DNA detected, No HIV-1 proviral DNA detected, or Indeterminate
Limitations:

Does not detect HIV-2 virus. Patients undergoing CAR-T cell therapy may test positive with this assay in the absence of HIV infection.
Additional Tests Recommended:

Individuals testing positive should have HIV viral load testing, available at reference laboratories.
Additional Comments:

Additional testing is recommended for all patients testing positive for the first time.
Methodology:

Real-time PCR
Includes:

Qualitative detection of HIV-1 DNA
Regulatory Compliance:
CPT Code: 87535
Price: $276.00
Fee Exempt Eligible:

Yes, with DHS approval only, 608-267-9003 
Billing and Revenue Office:

1-800-862-1065 arbill@slh.wisc.edu
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