Dengue Fever, Serology
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | SS02272 |
| Day(s) Performed: | Test is performed once a week |
| Turn-around Time: | 2-7 days |
| Recommended Uses: | Serodiagnosis of individuals with clinical symptoms of acute dengue fever or dengue hemorrhagic fever. Specimens should be collected in the acute phase of illness. |
| Pre-approval: | No |
| Contraindications: | Testing should not be performed as a screening test or on patients without symptoms of a dengue infection. |
| Additional Tests Performed: |
All Presumptive Positive or Equivocal specimens will be automatically sent to CDC for confirmatory testing (fee-exempt). |
| Preparation for Collection: | |
| Specimen Requirements: | 1-3 ml serum or 8.5 ml SST vacutainer tube, no additives |
| Collection Instructions: | Routine blood draw |
| Collection Kit/Container: |
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| Requisition Form: |
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| Required Information: | Clinical information regarding symptoms and onset must be provided. Patients should have recent travel history to a tropical area or to areas that have previously experienced dengue outbreaks. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers. |
| Rejection Criteria: | Hemolyzed or contaminated samples are not acceptable for serology testing. Specimens received without cold packs or dry ice. Specimens collected post mortem are unacceptable for testing. |
| Specimen Handling: | Specimens should be stored at 2-8°C, transported with a frozen cold pack, and should be received within 48 hours of collection. If specimens will not be tested for 3 or more days, they should be frozen at -20°C until ready for shipment and shipped on dry ice. |
| Packaging Requirements: | |
| Courier Information: | This test is not eligible for fee-exempt courier service. |
| Specimen Receiving Hours: | Monday-Friday 6:00 AM - 4:30 PM, Saturday 6:30 AM - 12:30 PM |
| Results Include: | Negative, Equivocal, Presumptive Positive |
| Limitations: | Serological cross-reactivity across the flavivirus group may occur. Certain sera from patients infected with Zika, Japanese Encephalitis, West Nile, Saint Louis Encephalitis viruses and/or recent vaccinations for Yellow Fever Virus may give false positive results. Therefore, any Dengue positive sera must be confirmed with other tests. |
| Additional Tests Recommended: | CDC recommends also testing by PCR (SS02310) to increase the likelihood of detecting Dengue. |
| Additional Comments: | Assay results should be interpreted only in the context of other laboratory findings and the total clinical state of the patient. |
| Methodology: | IgM Antibody Capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA) |
| Includes: | Qualitative IgM testing for Dengue Virus IgM antibodies |
| Regulatory Compliance: |
| CPT Code: | 86790 |
| Price: | $154.00 |
| Fee Exempt Eligible: | Yes, with DHS approval only, 608-267-9003 |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |