Dengue Fever, Serology
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | SS02272 |
| Day(s) Performed: | Test is performed once a week |
| Turn-around Time: | 2-7 days |
| Recommended Uses: | Serodiagnosis of individuals with clinical symptoms of acute dengue fever or dengue hemorrhagic fever. Specimens should be collected in the acute phase of illness. |
| Pre-approval: | N |
| Contraindications: | Testing should not be performed as a screening test or on patients without symptoms of a dengue infection. |
| Additional Tests Performed: |
All Presumptive Positive or Equivocal samples will automatically be sent to CDC and confirmed by Plaque Reduction Neutralization Test (PRNT) or by using the latest CDC guideline for diagnosis of this disease. |
| Preparation for Collection: | |
| Specimen Requirements: | 1-3 ml serum or 8.5 ml SST vacutainer tube, no additives |
| Collection Instructions: | Routine blood draw |
| Collection Kit/Container: |
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| Requisition Form: |
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| Required Information: | Clinical information regarding symptoms and onset must be provided. Patients should have recent travel history to a tropical area or to areas that have previously experienced dengue outbreaks. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Rejection Criteria: | Hemolyzed or contaminated samples are not acceptable for serology testing. Specimens received without cold packs or dry ice. Specimens collected post mortem are unacceptable for testing. |
| Specimen Handling: | Specimens should be stored at 2-8 °C, transported with a frozen cold pack, and should be received within 48 hours of collection. If specimens will not be tested for 3 or more days, they should be frozen at -20°C until ready for shipment and shipped on dry ice. |
| Packaging Requirements: | |
| Courier Information: | This test is not eligible for fee-exempt courier service. |
| Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Results Include: | Negative, Equivocal, Presumptive Positive |
| Limitations: | Serological cross-reactivity across the flavivirus group may occur. Certain sera from patients infected with Zika, Japanese Encephalitis, West Nile, Saint Louis Encephalitis viruses and/or recent vaccinations for Yellow Fever Virus may give false positive results. Therefore, any Dengue positive sera must be confirmed with other tests. |
| Additional Tests Recommended: | CDC recommends also testing by PCR (SS02310) to increase the likelihood of detecting Dengue. |
| Additional Comments: | Assay results should be interpreted only in the context of other laboratory findings and the total clinical state of the patient. |
| Methodology: | IgM Antibody Capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA) |
| Includes: | Qualitative IgM testing for Dengue Virus IgM antibodies |
| Regulatory Compliance: |
| CPT Code: | 86790 |
| Price: | $154.00 |
| Fee Exempt Eligible: | Yes, with DHS approval only, 608-267-9003 |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |