Arbovirus IgM Antibody Panel
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| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | SS02201 |
| Day(s) Performed: | Test performed weekly |
| Turn-around Time: | 3-14 days |
| Recommended Uses: | Serodiagnosis of a recent infection with Eastern Equine Encephalitis, St. Louis Encephalitis, La Crosse Encephalitis, West Nile Virus, Powassan Virus and Jamestown Canyon Virus in individuals with symptoms of arbovirus encephalitis and no other laboratory diagnosis. |
| Pre-approval: | N |
| Contraindications: | Testing will not be performed as a screening test or on patients without symptoms of arbovirus infection. |
| Additional Tests Performed: |
| Preparation for Collection: | Specimens should be collected in the acute phase of illness. |
| Specimen Requirements: | 1-3 ml serum or 8.5 ml SST vacutainer tube, no additives; CSF, minimum 2 ml. CSF must be accompanied by serum specimen. |
| Collection Instructions: | Routine blood draw |
| Collection Kit/Container: |
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| Requisition Form: |
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| Required Information: | Clinical information regarding symptoms and onset must be provided. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Rejection Criteria: | Specimens received without cold packs or dry ice. Specimens collected post mortem are unacceptable for testing. |
| Specimen Handling: | Specimens should be stored at 2-8 °C, transported with a frozen cold pack, and should be received within 48 hours of collection. If specimens will not be tested for 3 or more days, they should be frozen at -20°C until ready for shipment and shipped on dry ice. |
| Packaging Requirements: | |
| Courier Information: | This test is not eligible for fee-exempt courier service. |
| Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Results Include: | Negative, Presumptive Positive or Nonspecific for MIA assay. |
| Limitations: | Cross-reaction may occur between the arboviruses. Negative results on a single acute phase specimen do not rule out infection, as specimen may have been obtained prior to the development of an antibody response. |
| Additional Tests Recommended: | |
| Additional Comments: |
| Methodology: | Qualitative IgM capture ELISA for JCV, POWV and LAC; Microsphere immunoassay (MIA) for WNV/SLE and EEE |
| Includes: | Detection of IgM antibodies to Eastern Equine Encephalitis, St. Louis Encephalitis, La Crosse Encephalitis, West Nile Virus, Powassan Virus and Jamestown Canyon Virus in serum or CSF. |
| Regulatory Compliance: |
| CPT Code: | 86790x2, 86651, 86652, 86653, 86788 |
| Price: | $697.00 |
| Fee Exempt Eligible: | Yes, with DHS approval only, 608-267-9003 |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |