Eastern Equine Encephalitis (EEE) IgM Antibody !!! NOTE: The contents are valid only for: 05/09/2025 !!!
| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | SS02291 |
| Day(s) Performed: | Test is performed once a week |
| Turn-around Time: | 3-14 days |
| Recommended Uses: | Serodiagnosis of a recent infection with Eastern Equine Encephalitis virus (EEE) in individuals with symptoms of arbovirus infection. |
| Pre-approval: | N |
| Contraindications: | Testing should not be performed as a screening test or on patients without symptoms of EEE infection. |
| Additional Tests Performed: | |
| Preparation for Collection: | |
| Specimen Requirements: | 1-3 ml serum or 8.5 ml SST vacutainer tube, no additives; CSF, minimum 200 µl. CSF must be accompanied by serum specimen. |
| Collection Instructions: | Routine blood draw |
| Collection Kit/Container: | |
| Requisition Form: | |
| Required Information: | Clinical information regarding symptoms and onset must be provided. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Rejection Criteria: | Specimens received without cold packs or dry ice. Specimens collected post mortem are unacceptable for testing. |
| Specimen Handling: | Specimens should be stored at 2-8 °C, transported with a frozen cool pack, and should be received within 48 hours of collection. If specimens will not be tested for 3 or more days, they should be frozen at -20°C until ready for shipment and shipped on dry ice. |
| Packaging Requirements: | |
| Courier Information: | This test is not eligible for fee-exempt courier service |
| Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Results Include: | ** Negative: Test on single acute phase CSF or serum does not rule out infection. Lack of serological evidence of infection may reflect testing of acute phase specimen(s) obtained before development of an antibody response. ** Presumptive Positive: Serologic evidence for recent infection with Eastern Equine Encephalitis. ** Non-Specific: It is recommended that a serum specimen be collected in 2-3 weeks for follow-up testing. |
| Limitations: | Cross-reaction may occur between arboviruses. Negative results on a single acute phase specimen do not rule out infection, as specimen may have been obtained prior to the development of an antibody response. |
| Additional Tests Recommended: | Testing for other individual arboviral agents (LAC, WNV, POW, SLE, JC) is recommended. |
| Additional Comments: | |
| Methodology: | Microsphere Immunoassay (MIA) |
| Includes: | Qualitative IgM testing for Eastern Equine Encephalitis. |
| Regulatory Compliance: | |
| CPT Code: | 86652 |
| Price: | $93.00, Additional shipping charges may incur based on the algorithm |
| Fee Exempt Eligible: | Yes, with DHS approval only, 608-267-9003 |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |