Arbovirus RT-PCR Panel (Dengue, Zika, Chikungunya) !!! NOTE: The contents are valid only for: 12/27/2024 !!!
| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | SS02310 |
| Day(s) Performed: | Daily, Monday- Friday |
| Turn-around Time: | 3-5 days |
| Recommended Uses: | Patient presenting with clinical signs of acute flavivirus infection, including 2-7 days of fever, accompanied by headache, fever, retro-orbital eye pain, joint pain, muscle and/or bone pain, rash, and mild bleeding manifestations. Patient should also have recent travel history to an endemic area, or to areas that have previously experienced flavivirus outbreaks or have competent vectors. Patient samples must be taken during the acute phase of the disease (first 7 days of symptoms). |
| Contraindications: | Lack of febrile illness or signs/symptoms of infection. Lack of travel history to an endemic area or an area with competent vectors. |
| Additional Tests Performed: | |
| Patient Preparations: | Routine blood draw |
| Specimen Requirements: | � Serum 1-3 mL optimum, 0.5 mL minimum. Tube should be centrifuged prior to shipping to avoid hemolysis. � Cerebrospinal fluid 1-3 mL � Urine (Zika only) must be accompanied by serum |
| Specimen Handling & Transport: | Ship frozen serum specimens on dry ice overnight to the WSLH. |
| Collection Kit/Container: |
Serology Specimens
|
| Collection Instructions: | Freeze specimens (-20 to -80 C) and ship on dry ice. Sample should be taken during the acute phase of the disease (first 7 days of symptoms). |
| Unacceptable Conditions: | Specimen collected in container other than red-top or serum separator tube. Specimen stored or shipped at ambient temperatures. Serum hemolysis. Samples received more than 90 days after the onset of symptoms. |
| Requisition Form: |
CDD Requisition Form B
|
| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Dengue Virus DNA detected. No Dengue virus DNA detected. Chikungunya Virus RNA detected. No Chikungunya Virus RNA detected. Zika Virus RNA detected. No Zika Virus RNA detected. Inconclusive |
| Limitations: | False-negative results can arise from specimen collection after viral nucleic acid falls below the limit of detection of the assay (approximately 7 days post- onset of symptoms). Negative results do not preclude infection with these viruses and should not be used as the sole basis of a patient treatment/management decision. |
| Additional Tests Recommended: | CDC recommends also using a Dengue IgM antibody assay (SS02272) to increase the likelihood of diagnosing a dengue infection. |
| Additional Comments: | The U.S. Food and Drug Administration issued an Emergency Use Authorization for this assay and its performance characteristics have been verified at WSLH. Specimens positive for Dengue virus will be automatically sent to CDC for serotyping at no additional charge. |
| Methodology: | Real-time PCR |
| Includes: | Real-time PCR for the detection of dengue virus, Zika virus, and chikungunya virus RNA in serum and for the detection of Zika virus RNA in urine. CSF would be forwarded to the CDC. |
| CPT Code: | 87798 x3 (panel), 87798 (urine Zika) |
| Price: | $343.00 Price Effective 1/1/25 |