Streptococcus pneumoniae Characterization !!! NOTE: The contents are valid only for: 10/09/2026 !!!
| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP00689 |
| Day(s) Performed: | Monday-Friday |
| Turn-around Time: | 10-14 days |
| Recommended Uses: | To serotype strains of Streptococcus pneumoniae associated with invasive disease for public health surveillance |
| Pre-approval: | No |
| Contraindications: | Non-invasive isolates |
| Additional Tests Performed: |
Antimicrobial susceptibility testing. Please note antimicrobial susceptibility testing is performed for surveillance purposes only; no report will be provided. |
| Preparation for Collection: | |
| Specimen Requirements: | All Streptococcus pneumoniae isolates recovered from Cerebrospinal Fluid (CSF) Invasive Streptococcus pneumoniae isolates from patients that are 18 years and younger that meet at least one of the following: Non-susceptible to any clinically relevant antibiotic according to current CLSI M100 guidance Suspected vaccine failure Suspected treatment failure Suspected outbreak Please see the WSLH Laboratory-Based Surveillance Plan for detailed information. |
| Collection Instructions: | |
| Collection Kit/Container: |
Isolate on chocolate agar or blood agar |
| Requisition Form: |
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| Required Information: | Please include all pertinent clinical information including vaccination history and a copy of susceptibility testing results, if performed. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers. |
| Rejection Criteria: | Isolates that do not meet established surveillance criteria |
| Specimen Handling: | Isolates may be stored and transported at ambient temperatures |
| Packaging Requirements: | |
| Courier Information: | Specimens submitted for public health surveillance are eligible for fee-exempt courier service. Use the Purple Mountain portal for specimen pick up (in Wisconsin) or use the VPD FedEx account if sending from out of state. |
| Specimen Receiving Hours: | Monday-Friday 6:00 AM - 4:30 PM, Saturday 8:00 AM - 12:00 PM |
| Results Include: | Streptococcus pneumoniae serotype |
| Limitations: | Cultures must be viable for identification. Mixed cultures may significantly lengthen turnaround time. It may not always be possible to discriminate between multiple serotypes within a serogroup due to a high degree of genetic similarities or due to the existence of DNA polymorphisms among single serotypes. Therefore, a serogroup will be assigned for isolates where a serotype cannot be identified. Isolates that are not able to be serotyped may be sent to a reference lab for further identification at no additional charge. |
| Additional Tests Recommended: | |
| Additional Comments: | One positive isolate per patient per year is requested for public heath surveillance. |
| Methodology: | Whole Genome Sequencing |
| Includes: | Bacterial isolate identification confirmation, genotyping/serotyping of isolates |
| Regulatory Compliance: | |
| CPT Code: | 87999 |
| Price: | $133.00 |
| Fee Exempt Eligible: | Yes, for isolates meeting the public health surveillance request |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |