Dengue Fever, PCR and Serotyping_INTERNAL ONLY !!! NOTE: The contents are valid only for: 03/23/2025 !!!
| WSLH Department: | CDD - Sendouts and Referred Testing |
| WSLH Test Code: | SS02772 |
| Day(s) Performed: | Daily, Monday- Friday |
| Turn-around Time: | 12 days |
| Recommended Uses: | This test aids in the diagnosis of acute dengue virus infection in patients presenting with clinical signs and symptoms. Symptoms include 2-7 days of fever, often accompanied by headache, retro-orbital eye pain, joint pain, muscle and/or bone pain, rash, and mild bleeding manifestations. Patient should also have recent travel history to a tropical area or to an area with DENV-competent vectors and/or has previously experienced dengue infection. A blood sample taken during the first 7 days of symptoms is required. |
| Pre-approval: | N |
| Contraindications: | Lack of febrile illness or signs/symptoms of dengue infection. Lack of travel history to an endemic area or an area with DENV-competent vectors. Specimen collected more than 7 days after onset of symptoms. |
| Additional Tests Performed: | |
| Preparation for Collection: | Routine blood draw |
| Specimen Requirements: | Freeze at -20 degrees C until shipped. Avoid freeze-thaw cycles. |
| Collection Instructions: | Sample must be taken during the acute phase of the disease (first 7 days of symptoms). |
| Collection Kit/Container: |
Serology Specimens
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| Requisition Form: |
CDD Requisition Form B
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Rejection Criteria: | Specimen collected in container other than red-top or serum separator tube. Specimen stored or shipped at ambient temperatures. Serum hemolysis. Samples received more than 90 days after the onset of symptoms. |
| Specimen Handling: | Ship serum specimens overnight to the WSLH. Ship frozen specimens on dry ice. |
| Packaging Requirements: | |
| Courier Information: | |
| Specimen Receiving Hours: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Results Include: | |
| Limitations: | A negative result does not rule out infection with dengue virus. |
| Additional Tests Recommended: | |
| Additional Comments: | |
| Methodology: | Send out to CDC for testing |
| Includes: | Made Internal ONLY on 7/9/24 Real-time PCR and ELISA for the detection and serotype identification of dengue virus |
| Regulatory Compliance: | |
| CPT Code: | |
| Price: | $62.00 |
| Fee Exempt Eligible: | N |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |