Myeloma FISH panel !!! NOTE: The contents are valid only for: 12/01/2024 !!!
| WSLH Department: | Cytogenetics |
| WSLH Test Code: | |
| Day(s) Performed: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | Approximately 4-8 days, with an average of 6 days. (Reports are issued Monday-Friday 7:45 Am - 4:30 PM) |
| Recommended Uses: | Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant. May be performed in conjunction with other FISH assays, chromosome analysis or as an independent test. |
| Contraindications: | |
| Additional Tests Performed: | |
| Patient Preparations: | |
| Specimen Requirements: | 0.5-1.0 ml early aspirate bone marrow OR 1-2 ml whole blood collected in sodium heparin vacuum type tube |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 to 48 hours of collection. |
| Collection Kit/Container: | |
| Collection Instructions: | **Bone Marrow: 0.5-1.0ml bone marrow from 1st or 2nd aspirate. Material submitted for cytogenetic analysis must be from an early aspirate to avoid dilution with blood. Draw sodium heparin solution (1000 USP units/ml) into the syringe to be used for aspiration and then expel (over-heparinization is toxic to the cells). Transfer the marrow to a sterile, Na-Heparin vacuum type tube for delivery to State Lab. If an aspirate is unsuccessful, a 10mm bone core biopsy may be submitted in a sterile transport medium with Na-Heparin. **Blood: Draw blood using aseptic techniques into a sterile sodium heparin vacuum type tube. Invert tube to mix. If using larger tubes, draw full tube of blood to avoid over-treatment with anti-coagulant. |
| Unacceptable Conditions: | Specimen that is clotted or hemolyzed is not acceptable. Specimen must not be frozen. Plasma and serum are not acceptable. |
| Requisition Form: |
Cytogenetics Lab Neoplasia Diagnosis Form #132
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. |
| Results Include: | Abnormalities detected/not detected. Result written using current International System for Human Cytogenetic Nomenclature (ISCN) and interpretation of results. |
| Limitations: | Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen. |
| Additional Tests Recommended: | |
| Additional Comments: | If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen. **If IGH gene rearrangement is not detected, we recommend changing to an abridged myeloma panel which does not include IGH fusion probes (CCND1/IGH, FGFR3/IGH, IGH/MAF, IGH/MAFB). |
| Methodology: | Fluorescence in situ hybridization performed on interphase nuclei. This test will be performed on interphase nuclei selected for CD138+ plasma cells using the EasySep Human CD138 Positive Selection Kit from StemCell Technologies if sample volume and cell numbers are adequate, otherwise it will be performed on unselected cells from whole bone marrow. |
| Includes: | FISH analysis for; 11q22 deletion/duplication, ATM gene; Deletion 13q14, D13S319; Deletion 17p13.1, TP53; Gain 1q; IgH Rearrangement; CCND1/IGH Fusion t(11;14)(q13;q11.2); FGFR3/IgH Fusion t(4;14)(p16;q32); IgH/MAF Fusion t(14;16)(q31;q23); IgH/MAFB Fusion t(14;20)(q32;q12) |
| CPT Code: | 88271x16, 88275x9 |
| Price: | For pricing information, please call 608-262-0402. |