Bordetella pertussis/parapertussis PCR (also detects B. holmesii) !!! NOTE: The contents are valid only for: 06/28/2025 !!!
| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | MP00311 |
| Day(s) Performed: | 3 times weekly - Monday, Wednesday, Friday |
| Turn-around Time: | 1-3 days |
| Recommended Uses: | Diagnosis of Bordetella pertussis (whooping cough) and Bordetella parapertussis. |
| Pre-approval: | No |
| Contraindications: | Asymptomatic individuals |
| Additional Tests Performed: |
|
| Preparation for Collection: | |
| Specimen Requirements: | Dry nasopharyngeal swabs, or nasopharyngeal aspirates. |
| Collection Instructions: | Dacron Swab or NP aspirate submitted in sterile, dry tube. |
| Collection Kit/Container: |
|
| Requisition Form: |
|
| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers. |
| Rejection Criteria: | Specimens should NOT be submitted in charcoal-based media or on wooden shaft swabs. Do NOT use cotton swabs. |
| Specimen Handling: | Specimens should be transported to the laboratory with frozen cold packs. |
| Packaging Requirements: | |
| Courier Information: | Only eligible for fee-exempt courier for outbreaks with health department pre-approval |
| Specimen Receiving Hours: | Monday-Friday 6:00 AM - 4:30 PM, Saturday 6:30 AM - 12:30 PM |
| Results Include: | B. pertussis, B. parapertussis or B. holmesii DNA detected/ not detected |
| Limitations: | Test results must be correlated with patient history and clinical presentation to confirm as a case of B. pertussis or B. parapertussis infection. A negative result does not rule out infection with B. pertussis or B. parapertussis. B. holmesii is an uncommon cause of respiratory illness in humans. |
| Additional Tests Recommended: | |
| Additional Comments: | |
| Methodology: | RT-PCR |
| Includes: | RT-PCR assay for the detection of Bordetella pertussis, Bordetella parapertussis and Bordetella holmesii DNA in respiratory specimens. |
| Regulatory Compliance: | |
| CPT Code: | 87798x3 |
| Price: | $174.99 |
| Fee Exempt Eligible: | Yes, with DHS approval only, 608-267-9003 |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |