Tissue Culture and Shipment !!! NOTE: The contents are valid only for: 12/26/2024 !!!
| WSLH Department: | Cytogenetics |
| WSLH Test Code: | 860 |
| Day(s) Performed: | Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM |
| Turn-around Time: | 14-21 days for shipping cells. Test results are dependent on the testing facility. |
| Recommended Uses: | Obtaining cell culture to be tested using biochemical (metabolic), molecular (DNA), and protein identification assays. |
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| Additional Tests Performed: | |
| Patient Preparations: | |
| Specimen Requirements: | Skin biopsy: 1-2mm punch biopsy OR Amniotic fluid: 15-20ml amniotic fluid |
| Specimen Handling & Transport: | Store and transport specimens at room temperature (may transport with coolant during hot weather, >85 degrees F). DO NOT FREEZE. The laboratory must receive specimens within 24 to 48 hours of collection. |
| Collection Kit/Container: | |
| Collection Instructions: | Contact the laboratory (608-262-0402) to obtain desired collection kit. ** Skin biopsies: Skin should be cleaned using alcohol; do not use iodine or betadine as these will compromise the cell growth in culture. Do a 1-2mm punch biopsy that goes full depth through the epidermis into the sub-cutaneous fat. Place the specimen in a sterile tissue vial containing transport media. ** Amniotic fluid: Collect amniotic fluid under sterile, ultrasound guided conditions using a 22-gauge needle inserted through the uterine wall and into the amniotic cavity. Discard the first 1-2ml of amniotic fluid to minimize the possibility of maternal cell contamination, and dispense 15-20 ml of the remaining fluid into two or three sterile 15 ml centrifuge tubes. |
| Unacceptable Conditions: | Sample must not be frozen or processed with formalin. |
| Requisition Form: |
Cytogenetics Lab Genetic Diagnosis Form #131
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| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. **Forms for the reference lab must be completed and submitted with the specimen. |
| Results Include: | |
| Limitations: | |
| Additional Tests Recommended: | |
| Additional Comments: | |
| Methodology: | Aseptic culture of adherent cells from specimen. |
| Includes: | Preparation of a cell culture from specimen. Cultured cells are shipped to the selected reference laboratory for desired testing. |
| CPT Code: | 88233 |
| Price: | For pricing information, please call 608-262-0402. |