HIV-1 Proviral DNA PCR !!! NOTE: The contents are valid only for: 10/31/2025 !!!
| WSLH Department: | CDD - Communicable Disease Division |
| WSLH Test Code: | SS00091 |
| Day(s) Performed: | Daily, Monday - Friday |
| Turn-around Time: | 1-3 Days |
| Recommended Uses: | Approved for testing neonates whose mothers are HIV antibody positive or considered to be at high risk for HIV infection; patients with HIV Ag/Ab Combo positive and with negative or indeterminate Geenius results for HIV; patients suspected of acute HIV infection prior to seroconversion. |
| Pre-approval: | Please call the lab prior to submission to arrange for testing (800-862-1013). |
| Contraindications: | Not approved as a primary screening assay (except for neonates) |
| Additional Tests Performed: |
|
| Preparation for Collection: | |
| Specimen Requirements: | One 7 ml EDTA (lavender top) vacutainer of whole blood for adults. Greater than or equal to 400 ul EDTA (lavender top) vacutainer of whole blood for pediatrics. Store refrigerated at 2-8°C. Patient specimen must be received within 5 days of collection. |
| Collection Instructions: | Routine blood draw Comply with Wisconsin informed opt-out statutes. |
| Collection Kit/Container: |
|
| Requisition Form: |
|
| Required Information: | Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier, date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label which is required to have a minimum of two identifiers. |
| Rejection Criteria: | Specimen collected in container other than EDTA or ACD vacutainer tube. Specimens received >5 days after collection. |
| Specimen Handling: | Patient specimen must be received within 5 days of collection. Ship on frozen cold pack. |
| Packaging Requirements: | |
| Courier Information: | This test is not eligible for fee-exempt courier service. |
| Specimen Receiving Hours: | Monday-Friday 6:00 AM - 4:30 PM, Saturday 6:30 AM - 12:30 PM |
| Results Include: | HIV-1 proviral DNA detected, No HIV-1 proviral DNA detected, or Indeterminate |
| Limitations: | Does not detect HIV-2 virus. Patients undergoing CAR-T cell therapy may test positive with this assay in the absence of HIV infection. |
| Additional Tests Recommended: | Individuals testing positive should have HIV viral load testing, available at reference laboratories. |
| Additional Comments: | Additional testing is recommended for all patients testing positive for the first time. |
| Methodology: | Real-time PCR |
| Includes: | Qualitative detection of HIV-1 DNA |
| Regulatory Compliance: | |
| CPT Code: | 87535 |
| Price: | $276.00 |
| Fee Exempt Eligible: | Yes, with DHS approval only, 608-267-9003 |
| Billing and Revenue Office: | 1-800-862-1065 arbill@slh.wisc.edu |